caa a22 4500 39752210X CHVBK 20180308164635.0 cr unu---uuuuu 161202e199501 xx s 000 0 eng 10.1093/jxb/46.1.111 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.1.111 Relative water content and water potential of tissue 1 [Elektronische Daten] [J.C. Diaz-Pérez, K.A. Shackel, E.G. Sutter] Relative water content (RWC) and water potential as measured with the pressure chamber were evaluated as indicators of water status of tissue-cultured apple shoots and plantlets (shoots with roots). During the hydration required for RWC measurement, both water content and water potential exhibited the same hydration kinetics, indicating that 10 h were required for full hydration. Once full hydration was reached, shoot mass remained relatively constant. Moisture release characteristics were also constructed and the associated shoot and plantlet water relations parameters were estimated. Underin vitroconditions, both shoot and plantlet water potential were similar to the water potential of the culture medium in which they were grown. The moisture release characteristic of shoots and plantlets was consistent with that expected for typical plant tissues, and gave estimates of maximum modulus of elasticity (6.20±1.14 MPa), osmotic potential at saturation (−0.85 ± 0.10 MPa), osmotic potential at zero turgor (−1.16 ± 0.14 MPa) and RWC at zero turgor (78 ±2%) which were similar to values in the literature. Higher values of leaf conductance and RWC were found in shoots and plantlets placed at 95% RH (21 °C) compared to those at 90% RH. Plantlets had higher values of both conductance and RWC compared to shoots, suggesting thatin vitroroots are functional in water uptake. Relative water content was related to measures of physiological activity such as leaf conductance, and it was also easier to measure than water potential. Relative water content is suggested as a sound index of water status in tissue culture plants. © Oxford University Press Research Papers nationallicence Conductance nationallicence microculture nationallicence water status nationallicence water stress nationallicence Diaz-Pérez J.C. Department of Pomology, University of California, Davis, CA 95616, USA aut Shackel K.A. Department of Pomology, University of California, Davis, CA 95616, USA aut Sutter E.G. Department of Pomology, University of California Davis, CA 95616, USA aut Journal of Experimental Botany Oxford University Press 46/1(1995-01), 111-118 0022-0957 46:1<111 1995 46 exbotj https://doi.org/10.1093/jxb/46.1.111 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.1.111 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Diaz-Pérez J.C. Department of Pomology, University of California, Davis, CA 95616, USA aut NATIONALLICENCE 700 1- Shackel K.A. Department of Pomology, University of California, Davis, CA 95616, USA aut NATIONALLICENCE 700 1- Sutter E.G. Department of Pomology, University of California Davis, CA 95616, USA aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/1(1995-01), 111-118 0022-0957 46:1<111 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford