caa a22 4500 397522118 CHVBK 20180308164635.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.9.1233 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.9.1233 Transformation of Antirrhinum majus using Agrobacterium rhizogenes [Elektronische Daten] [I. Senior, P. Holford, R.N. Cooley, H.J. Newbury] Hairy roots were produced following the co-cultivation of Agrobacterium rhizogenes cells with hypocotyls of five varieties of Antirrhinum majus. The use of a strain containing a binary plasmid with T-DNA bearing the β-glucuronidase reporter gene resulted in the co-transformation of some root clones. Regeneration of shoots from hairy roots occurred only with variety Golden Monarch. Regenerated plants, some of which were GUS-positive, exhibited the abnormal morphology common among hairy root regenerants; they were dwarfed, had an altered leaf shape, a poor root system and were very delayed in their flowering. Attempts to allow segregation of the two introduced T-DNAs during crossing of primary co-transformants with wild-type plants were not successful since all GUS-positive progeny possessed the abnormal morphology. However, ‘semi-dwarf' plants with morphology much more similar to wild-type were produced by the vegetative propagation of selected side-shoots from the transformants. © Oxford University Press RESEARCH PAPERS nationallicence Antirrhinum nationallicence Agrobacterium rhizogenes nationallicence transformation nationallicence hairy roots nationallicence Senior I. School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK aut Holford P. School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK aut Cooley R.N. School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK aut Newbury H.J. School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK aut Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1233-1239 0022-0957 46:9<1233 1995 46 exbotj https://doi.org/10.1093/jxb/46.9.1233 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.9.1233 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Senior I. School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK aut NATIONALLICENCE 700 1- Holford P. School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK aut NATIONALLICENCE 700 1- Cooley R.N. School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK aut NATIONALLICENCE 700 1- Newbury H.J. School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1233-1239 0022-0957 46:9<1233 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford