caa a22 4500 397522134 CHVBK 20180308164635.0 cr unu---uuuuu 161202e199501 xx s 000 0 eng 10.1093/jxb/46.1.95 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.1.95 Stomatal responses of Vicia faba L. to indole acetic acid and abscisic acid [Elektronische Daten] [P.J. Dunleavy, P.D. Ladley] Evidence is presented that stomata in isolated epidermal peels of Vicia faba L. open in darkness in response to the external presence of indole acetic acid (IAA) in the incubation medium. The effect of IAA is found to be overcome completely in the presence of either TRIS or MES buffers. In the absence of buffer, V. faba stomata are shown to be influenced by IAA in a concentration-dependent trend which reached a maximum at an [IAA] of 10−3 mol m−3. Further investigations reveal that stomata in this species can be shown to respond to the presence of IAA and another phytohormone, abscisic acid (ABA). IAA and ABA are demonstrated to be antagonistic in their effects provided the incubation conditions are suitable. The data are discussed in relation to stomatal responses of other species in different treatment conditions. Recommendations are made with respect to standardization of incubation media during epidermal peel experiments. © Oxford University Press Research Papers nationallicence Vicia faba nationallicence stomata nationallicence indole acetic acid nationallicence abscisic acid nationallicence buffers nationallicence Dunleavy P.J. The Manchester Metropolitan University, Department of Environmental and Geographical Sciences Manchester M15GD, UK aut Ladley P.D. University of Luton, Faculty of Applied Sciences Park Square, Luton, Bedfordshire LU13HZ, UK aut Journal of Experimental Botany Oxford University Press 46/1(1995-01), 95-100 0022-0957 46:1<95 1995 46 exbotj https://doi.org/10.1093/jxb/46.1.95 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.1.95 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dunleavy P.J. The Manchester Metropolitan University, Department of Environmental and Geographical Sciences Manchester M15GD, UK aut NATIONALLICENCE 700 1- Ladley P.D. University of Luton, Faculty of Applied Sciences Park Square, Luton, Bedfordshire LU13HZ, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/1(1995-01), 95-100 0022-0957 46:1<95 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford