caa a22 4500 397522185 CHVBK 20180308164636.0 cr unu---uuuuu 161202e199507 xx s 000 0 eng 10.1093/jxb/46.7.803 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.7.803 Electrical properties of intact pollen grains of Lilium longiflorum: characteristics of the non-germinatingpollen grain [Elektronische Daten] [G. Obermeyer, M.R. Blatt] As a first step to characterizing membrane function during the process of germination in pollen, the transport characteristics of Lilium longiflorum Thunbpollen were examined in the quiescent grains.Membrane voltages were recorded, and conventional voltage-clamp measurements were carried out with double-barrelled microelectrodes. The resting membrane voltage (Vm) was found to depend on the external K+; concentration and generally followed the equilibrium potential for Kplus; (Ek). In some cells a more negative Vm was measured indicating the presence of an electrogenic pump. The presence of a pump current was also detected by a depolarization of the plasma membrane after inhibition of ATP synthesis by addition of CN− and SHAM. Besides this pump, two other current components were found in the plasma membrane of ungerminated pollen grains: an outward potassium current (lk,out) and an inward K+ current (lk,in). Outward K+ currents were detected at membrane voltages more positive than the resting voltage and were blocked by externally applied TEA+ and Ba2+. The voltage at which lk,out was first detected shifted in parallel with the equilibrium potential for K+. By contrast, activation of lk,in was less affected by the external K+ concentration, except that the magnitude of the inward current increased with K+ concentration. The detected current components may be involved in initiation of osmotic water influx for germination by allowing a K+ influx after the membrane voltage has been driven more negative than Ek by an electrogenic pump. © Oxford University Press Research Papers nationallicence Pollen nationallicence germination nationallicence potassium channel nationallicence proton pump nationallicence voltage-clamp nationallicence Obermeyer G. Institut für Pflanzenphysiologie, Universität Salzburg Hellbrunner Strasse 34, 5020 Salzburg, Austria aut Blatt M.R. Department of Biological Sciences, Wye College, University of London Wye, Ashford, Kent TN25 5AH, UK aut Journal of Experimental Botany Oxford University Press 46/7(1995-07), 803-813 0022-0957 46:7<803 1995 46 exbotj https://doi.org/10.1093/jxb/46.7.803 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.7.803 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Obermeyer G. Institut für Pflanzenphysiologie, Universität Salzburg Hellbrunner Strasse 34, 5020 Salzburg, Austria aut NATIONALLICENCE 700 1- Blatt M.R. Department of Biological Sciences, Wye College, University of London Wye, Ashford, Kent TN25 5AH, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/7(1995-07), 803-813 0022-0957 46:7<803 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford