caa a22 4500 397522193 CHVBK 20180308164636.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/jxb/46.6.687 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.6.687 The role of abscisic acid in germination, storage protein synthesis and desiccation tolerance in alfalfa (Medicago sativa L.) seeds, as shown by inhibition of its synthesis by fluridone during development [Elektronische Daten] [Nanfei Xu, J. Derek Bewley] Abscisic acid and osmoticum maintain maturation and protein synthesis of developing alfalfa embryos, individually and in combination. The in situ environment of developing alfalfa zygotic embryos is rich in ABA and low in osmotic potential. When ABA synthesis was inhibited by treating the pods with fluridone at an early stage of development, the seeds which subsequently developed contained low amounts of ABA, but had a similar osmotic potential as untreated control seeds. The reduced ABA in seeds from fluridone-treated pods did not change the morphology except the colour of seeds, nor did it induce viviparous germination or affect storage protein synthesis. However, two nonstorage proteins which were synthesized in control seeds during early to mid-development were absent from fluridone-treated seeds. Control seeds containing these two proteins were desiccation-tolerant, whereas the fluridone-treated seeds which lacked them were desiccation-intolerant, at least until the deposition of storage proteins was nearly complete. Culture of isolated embryos on nutrient medium induced germination and curtailed storage protein synthesis in the embryos. Addition of either ABA or osmoticum to the nutrient medium prevented germination and maintained storage protein synthesis. When fluridone was added along with osmoticum, germination occurred, but storage protein synthesis was maintained. © Oxford University Press Research Papers nationallicence Embryogenesis nationallicence Medicago sativa L nationallicence alfalfa nationallicence ABA nationallicence osmotic potential nationallicence fluridone nationallicence desiccation nationallicence storage protein synthesis nationallicence Xu Nanfei Department of Botany, University ofGuelph, Guelph, Ontario N1G 2W1, Canada aut Bewley J. Derek Department of Botany, University ofGuelph, Guelph, Ontario N1G 2W1, Canada aut Journal of Experimental Botany Oxford University Press 46/6(1995-06), 687-694 0022-0957 46:6<687 1995 46 exbotj https://doi.org/10.1093/jxb/46.6.687 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.6.687 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Nanfei Department of Botany, University ofGuelph, Guelph, Ontario N1G 2W1, Canada aut NATIONALLICENCE 700 1- Bewley J. Derek Department of Botany, University ofGuelph, Guelph, Ontario N1G 2W1, Canada aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/6(1995-06), 687-694 0022-0957 46:6<687 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford