caa a22 4500 397522312 CHVBK 20180308164636.0 cr unu---uuuuu 161202e199512 xx s 000 0 eng 10.1093/jxb/46.12.1809 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.12.1809 Anatomy, physiology and biochemistry of root nodules of Sprint-2 Fix−, a symbiotically defective mutant of pea (Pisum sativum L.) [Elektronische Daten] [V.I. Romanov, A.J. Gordon, F.R. Minchin, J.F. Witty, L. Skøt, C.L. James, A.Y. Borisov, I.A. Tikhonovich] A plant-determined pea mutant Sprint-2 Fix− and the parental line Sprint-2 were compared for selected physiological and biochemical parameters. The Fix− mutation prevented differentiation of Rhizobium leguminosarum bacteria into bacteroids and produced large, white, non-fixing nodules. These lacked nitrogenase-linked respiration, but had a background rate of CO2 evolution similar to the normal Fix+ nodules. The cortical structure of the ineffective nodules suggests the existence of an oxygen diffusion barrier and this was supported by a low oxygen concentration in the central region (0.5-3.0%), measured using an O2 sensitive micro-electrode. Sucrose and starch contents were similar in normal and ineffective nodules while ononitol content was about 15 times lower in the Fix− nodules. The distribution of starch was also different in the two nodule types. The activities of glutamine synthetase (GS), sucrose synthase (SS), phosphoenolpyruvate carboxylase (PEPC) and alanine pyruvate aminotransferase (APAT) were markedly higher in Fix+ nodules while the activities of pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH) and glutamate dehydrogenase (GDH) were higher in Fix− nodules. The data from immunodetection of host nodule proteins confirmed the reduced levels of sucrose synthase and the almost complete absence of glutamine synthetase and leghaemoglobin in mutant nodules. There was no significant difference in the amount of nitrogenase component 1 extracted from the microsymbiont of normal and ineffective nodules, but component 2 was hardly detectable in the Fix− mutant. © Oxford University Press Research Papers nationallicence Pisum sativum nationallicence Fix− mutant nationallicence nodules nationallicence Romanov V.I. A.N. Bach Institute of Biochemistry Leninsky Prospect 33, Moscow 117071, Russia aut Gordon A.J. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut Minchin F.R. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut Witty J.F. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut Skøt L. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut James C.L. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut Borisov A.Y. All-Russian Institute of Agricultural Microbiology St Petersburg-Pushkin, Shosse Podbelsky 3, 188620, Russia aut Tikhonovich I.A. All-Russian Institute of Agricultural Microbiology St Petersburg-Pushkin, Shosse Podbelsky 3, 188620, Russia aut Journal of Experimental Botany Oxford University Press 46/12(1995-12), 1809-1816 0022-0957 46:12<1809 1995 46 exbotj https://doi.org/10.1093/jxb/46.12.1809 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.12.1809 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Romanov V.I. A.N. Bach Institute of Biochemistry Leninsky Prospect 33, Moscow 117071, Russia aut NATIONALLICENCE 700 1- Gordon A.J. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut NATIONALLICENCE 700 1- Minchin F.R. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut NATIONALLICENCE 700 1- Witty J.F. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut NATIONALLICENCE 700 1- Skøt L. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut NATIONALLICENCE 700 1- James C.L. Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY233EB, UK aut NATIONALLICENCE 700 1- Borisov A.Y. All-Russian Institute of Agricultural Microbiology St Petersburg-Pushkin, Shosse Podbelsky 3, 188620, Russia aut NATIONALLICENCE 700 1- Tikhonovich I.A. All-Russian Institute of Agricultural Microbiology St Petersburg-Pushkin, Shosse Podbelsky 3, 188620, Russia aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/12(1995-12), 1809-1816 0022-0957 46:12<1809 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford