caa a22 4500 397522444 CHVBK 20180308164636.0 cr unu---uuuuu 161202e199501 xx s 000 0 eng 10.1093/jxb/46.1.27 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.1.27 Chimeric nature of precociously-germinating Brassica napus embryos: mRNA accumulation patterns [Elektronische Daten] [Sherryl R. Bisgrove, Martha L. Crouch, Donna E. Fernandez] During precocious germination, Brassica napus L. embryos exhibit features of embryo maturation and features of post-germinative growth at the same time, which raises important questions about the nature of these developmental programmes and the transition between them. To investigate the possiblity that the two programmes are segregated spatially rather than temporallhy in these embryos, embryos of different ages (excised at 38, 47, and 60 d post-anthesis) were placed in culture to induce precocious germination, and totoal RNA was isolated from the cotyledons and axes or hypocotyls. cDNA sequences (two marking embryo maturation and four marking the onset of post-germinative growth) were used as probes on dot blots to compare mRNA population dynamics in these organs. We find that individual organs accumulate mRNAs characteristic of only one developmental programme at any given time and conclude that the programmes are both distinct and potentially mutually exclusive. Embryos are able to ‘switch' from accumulating embryo maturation mRNAs to acumulating post-germinative mRNAs in culture, but the transition occurs at different times in different organs. © Oxford University Press Research Papers nationallicence Precocious germination nationallicence Brassica napus nationallicence rapeseed nationallicence embryo development nationallicence Bisgrove Sherryl R. Department of Biology, Indiana University Bloomington, IN 47405, USA aut Crouch Martha L. Department of Biology, Indiana University Bloomington, IN 47405, USA aut Fernandez Donna E. Department of Botany, University of Wisconsin, 430 Lincoln Drive, Madison WI 53706-1381, USA aut Journal of Experimental Botany Oxford University Press 46/1(1995-01), 27-33 0022-0957 46:1<27 1995 46 exbotj https://doi.org/10.1093/jxb/46.1.27 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.1.27 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bisgrove Sherryl R. Department of Biology, Indiana University Bloomington, IN 47405, USA aut NATIONALLICENCE 700 1- Crouch Martha L. Department of Biology, Indiana University Bloomington, IN 47405, USA aut NATIONALLICENCE 700 1- Fernandez Donna E. Department of Botany, University of Wisconsin, 430 Lincoln Drive, Madison WI 53706-1381, USA aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/1(1995-01), 27-33 0022-0957 46:1<27 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford