caa a22 4500 397522517 CHVBK 20180308164636.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jxb/46.2.231 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.2.231 Ultrastructural localization of a diprenylated isoflavone in Rhizobium lupini-Lupinus albus symbiotic association [Elektronische Daten] [Jacques Grandmaison, Ragai Ibrahim] Polyclonal IgGs raised against the diprenylated isoflavone, 2′-hydroxylupalbigenin were used in an immunocytochemical study of the root and nodule tissues of white lupin (Lupinus albus L.). In the roots, the antigen was detected in the secondary walls of both xylem vessels and pericycle cells. Examination of nodules revealed the presence of the antigen in the inner cortex, associated with a neoparietal material which is deposited as globules on the cell wall and occluding the intercellular spaces. The discrete location of the diprenylated isoflavone in specialized cytoplasmic organelles suggests that its compartmentation within the wall is mediated by membrane vesicles. In the infected cells, more than half of the bacteroids exhibited a specific labelling of their inner core associated with a central fibrillar system, and sometimes surrounding an electron-dense nucleoid region. These results suggest that 2′-hydroxylupalbigenin, and possibly other prenylated isoflavones, may play an important role as a biochemical factor in early symbiotic events other than nod gene induction or inhibition. Its putative biological functions in the post-infectional phases of symbiosis are discussed. © Oxford University Press Research Papers nationallicence Bacteroid nationallicence 2′-hydroxylupalbigenin nationallicence in situ localization nationallicence Lupinus albus nationallicence nodules nationallicence Grandmaison Jacques Plant Biochemistry Laboratory, Department of Biology, Concordia University Montréal, Québec, Canada H3G 1M8 aut Ibrahim Ragai Plant Biochemistry Laboratory, Department of Biology, Concordia University Montréal, Québec, Canada H3G 1M8 aut Journal of Experimental Botany Oxford University Press 46/2(1995-02), 231-237 0022-0957 46:2<231 1995 46 exbotj https://doi.org/10.1093/jxb/46.2.231 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.2.231 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grandmaison Jacques Plant Biochemistry Laboratory, Department of Biology, Concordia University Montréal, Québec, Canada H3G 1M8 aut NATIONALLICENCE 700 1- Ibrahim Ragai Plant Biochemistry Laboratory, Department of Biology, Concordia University Montréal, Québec, Canada H3G 1M8 aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/2(1995-02), 231-237 0022-0957 46:2<231 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford