caa a22 4500 397522541 CHVBK 20180308164637.0 cr unu---uuuuu 161202e199504 xx s 000 0 eng 10.1093/jxb/46.4.377 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.4.377 Apoplastic pH of intact leaves of Vicia faba as influenced by light [Elektronische Daten] [K.H. Mühling, C. Plieth, U-P. Hansen, B. Sattelmacher] The fluorochrome FITC-dextran was used to measure the effect of light on the apoplastic pH of intact Vicia faba leaves with the ratio imaging technique. In darkadapted leaves the apoplastic pH varied depending on the leaf between 5.2 and 5.9. Red light (660 nm, 4-12 W m−2) leads to multiphasic responses: in the first seconds an alkalinization (˜0.3 pH units), and thereafter an acidification of the leaf apoplast (˜0.4 pH units) were observed. Both effects could be inhibited by DCMU. While variation of CO2 concentration revealed no effect on light-induced apoplastic pH changes, a decrease in O2 concentration decreased the effect. On the basis of our data it is suggested that the influence of photosynthesis on plasmalemma H+ ATPase is responsible for the observed effects, rather than altered CO2 uptake. © Oxford University Press Articles nationallicence Leaf apoplast nationallicence apoplastic pH nationallicence light nationallicence ratio imaging nationallicence pH-sensitive fluorescent dye nationallicence Vicia fab nationallicence Mühling K.H. Institute for Plant Nutrition and Soil Science, Christian-Albrechts University OlshausenstraBe 40, 24098 Kiel, Germany aut Plieth C. Institute for Applied Physics, Christian-Albrechts University OlshausenstraBe 40, 24098 Kiel, Germany aut Hansen U-P Institute for Applied Physics, Christian-Albrechts University OlshausenstraBe 40, 24098 Kiel, Germany aut Sattelmacher B. Institute for Plant Nutrition and Soil Science, Christian-Albrechts University OlshausenstraBe 40, 24098 Kiel, Germany aut Journal of Experimental Botany Oxford University Press 46/4(1995-04), 377-382 0022-0957 46:4<377 1995 46 exbotj https://doi.org/10.1093/jxb/46.4.377 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.4.377 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mühling K.H. Institute for Plant Nutrition and Soil Science, Christian-Albrechts University OlshausenstraBe 40, 24098 Kiel, Germany aut NATIONALLICENCE 700 1- Plieth C. Institute for Applied Physics, Christian-Albrechts University OlshausenstraBe 40, 24098 Kiel, Germany aut NATIONALLICENCE 700 1- Hansen U-P Institute for Applied Physics, Christian-Albrechts University OlshausenstraBe 40, 24098 Kiel, Germany aut NATIONALLICENCE 700 1- Sattelmacher B. Institute for Plant Nutrition and Soil Science, Christian-Albrechts University OlshausenstraBe 40, 24098 Kiel, Germany aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/4(1995-04), 377-382 0022-0957 46:4<377 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford