caa a22 4500 397522568 CHVBK 20180308164637.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.special_issue.1245 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.special_issue.1245 Is there another player in the game of Rubisco regulation? [Elektronische Daten] [Alfred J. Keys, Lain Major, Martin A.J. Parry] Leaves of French bean plants, whether collected in the dark or in the light, contained a tight-binding inhibitor of ribulose 1,5-bisphosphate carboxylase/ oxygenase (Rubisco). Wheat leaves contained such an inhibitor only in the light. The inhibitor in wheat leaves was not 2-carboxy-D-arabinitol 1-phosphate (CA1P) and not xylulose 1,5-bisphosphate. It was stable in acid conditions, but was destroyed during low pressure anion exchange chromatography and in alkaline conditions, especially during concentration of solutions. Although the kinetics of inhibition suggested tight-binding, the inhibitor did not survive attempts at purification by binding it to purified Rubisco followed by denaturation of the protein. The retention time of the inhibitory component on HPLC anion-exchange columns suggested that the substance was a sugar bisphosphate. Reduction with tritiated sodium borohydride and dephosphorylation produced radioactive sugar alcohols identified by TLC as ribitol and arabinitol. The small amounts of this inhibitor detected do not suggest a major physiological role, but instability during extraction may be obscuring its true importance. © 1995 Oxford University Press Plenary Papers nationallicence Inhinitors nationallicence regulation nationallicence Rubisco nationallicence sugar bisphosphates nationallicence Triticum aestivum nationallicence Keys Alfred J. Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK aut Major Lain Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK aut Parry Martin A.J. Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK aut Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1245-1251 0022-0957 46:special_issue<1245 1995 46 exbotj https://doi.org/10.1093/jxb/46.special_issue.1245 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.special_issue.1245 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keys Alfred J. Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK aut NATIONALLICENCE 700 1- Major Lain Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK aut NATIONALLICENCE 700 1- Parry Martin A.J. Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1245-1251 0022-0957 46:special_issue<1245 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford