caa a22 4500 397522614 CHVBK 20180308164637.0 cr unu---uuuuu 161202e199503 xx s 000 0 eng 10.1093/jxb/46.3.321 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.3.321 Phloem in developing cotton fruits: 6(5)carboxyfluorescein as a tracer for functional phloem [Elektronische Daten] [M.W. van lersel, W.M. Harris, D.M. Oosterhuis] Carbohydrate and water transport into developing cotton (Gossypium hirsutum L.) fruits is essential for normal fruit development, and phloem has been suggested to play a major role in this transport. To study transport into cotton fruits of different developmental stages, a phloem tracer [6(5)carboxyfluorescein] was applied to sympodial leaves. Tracer distribution into the subtended fruit was determined using epifluorescence microscopy. Phloem throughout the entire capsule wall and central column was functional from early stages of fruit development. Vasculature of the capsule wall consisted of a complex three-dimensional network. Vascular bundles ended in radially-oriented club-shaped structures, which consisted mainly of fibres, with phloem on the outside of these structures. The vascular system of the capsule wall was connected to the central column by amphicribral bundles in the partitions. The central column had two adaxial (ventral) vascular bundles per carpel. These vascular bundles branched into the placental region of the central column, and connected to the seeds via the funiculi. Amphicribral vascular bundles diverged from these adaxial bundles into the partition. The results of this study confirmed previous observations on the vascular anatomy of the capsule wall and describe the vascular anatomy of the central column of cotton fruits © Oxford University Press Research Papers nationallicence Cotton nationallicence Gossypium hirsutum L nationallicence phloem nationallicence fruit nationallicence carboxyfluorescein nationallicence vasculature nationallicence van lersel M.W. Department of Agronomy, University of Arkansas Fayetteville, AR 72701, USA aut Harris W.M. Department of Biological Sciences, University of Arkansas Fayetteville, AR 72701, USA aut Oosterhuis D.M. Department of Agronomy, University of Arkansas Fayetteville, AR 72701, USA aut Journal of Experimental Botany Oxford University Press 46/3(1995-03), 321-328 0022-0957 46:3<321 1995 46 exbotj https://doi.org/10.1093/jxb/46.3.321 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.3.321 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van lersel M.W. Department of Agronomy, University of Arkansas Fayetteville, AR 72701, USA aut NATIONALLICENCE 700 1- Harris W.M. Department of Biological Sciences, University of Arkansas Fayetteville, AR 72701, USA aut NATIONALLICENCE 700 1- Oosterhuis D.M. Department of Agronomy, University of Arkansas Fayetteville, AR 72701, USA aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/3(1995-03), 321-328 0022-0957 46:3<321 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford