caa a22 4500 397522665 CHVBK 20180308164637.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jxb/46.2.239 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.2.239 Improved protoplast yield and cell wall regeneration in Gracilaria verrucosa (Huds.) Papenfuss (Gracilariales, Rhodophyta) [Elektronische Daten] [J.C. Mollet, M.C. Verdus, R. Kling, H. Morvan] Protoplasts were isolated enzymatically from the red alga Gracilaria verrucosa using only two enzymes: agarase prepared from marine bacteria and commercial cellulase. Yields of protoplasts were dependent on the donor material and by choosing young blades or algae in a state of higher growth rate, the production of protoplasts reached a maximum of 107 protoplasts per gram of fresh tissue. Cell viability was better with NaCI used as osmoticum than with sorbitol in the culture medium and on reducing culture media to normal osmolarity in 4 d. 25% of the cultured protoplasts were able to regenerate a cell wall (i.e. cellulose) within 7 d as confirmed by staining with calcofluor white although only a few protoplasts were able to divide. During the first 24 h of culture, the synthesized agar contained higher amounts of L-galactose-6-sulphate than the cell wall of thalli. The amount of agar in the protoplasts, however, did not increase, indicating that the protoplasts synthesize a qualitatively differentcell wall. © Oxford University Press Research Papers nationallicence Agarase nationallicence agar nationallicence cell wall regeneration nationallicence Gracilaria verrucosa nationallicence protoplasts nationallicence Mollet J.C. Laboratoire de Cytophysiologie Végétale et de Phycologie Bat. SN2, USTL, Cité scientifique, F-59655 Villeneuve d'Ascq, France aut Verdus M.C. Laboratoire des Processus loniques Cellulaires, Université de Rouen F-74134 Mont Saint-Aignan, France aut Kling R. Laboratoire de Cytophysiologie Végétale et de Phycologie Bat. SN2, USTL, Cité scientifique, F-59655 Villeneuve d'Ascq, France aut Morvan H. Laboratoire de Biologie Cellulaire Végétale et Valorisation des Espèces Ligneuses, Université de Limoges 123 rue Albert Thomas, F-87060 Limoges, France aut Journal of Experimental Botany Oxford University Press 46/2(1995-02), 239-247 0022-0957 46:2<239 1995 46 exbotj https://doi.org/10.1093/jxb/46.2.239 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.2.239 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mollet J.C. Laboratoire de Cytophysiologie Végétale et de Phycologie Bat. SN2, USTL, Cité scientifique, F-59655 Villeneuve d'Ascq, France aut NATIONALLICENCE 700 1- Verdus M.C. Laboratoire des Processus loniques Cellulaires, Université de Rouen F-74134 Mont Saint-Aignan, France aut NATIONALLICENCE 700 1- Kling R. Laboratoire de Cytophysiologie Végétale et de Phycologie Bat. SN2, USTL, Cité scientifique, F-59655 Villeneuve d'Ascq, France aut NATIONALLICENCE 700 1- Morvan H. Laboratoire de Biologie Cellulaire Végétale et Valorisation des Espèces Ligneuses, Université de Limoges 123 rue Albert Thomas, F-87060 Limoges, France aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/2(1995-02), 239-247 0022-0957 46:2<239 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford