caa a22 4500 397522673 CHVBK 20180308164637.0 cr unu---uuuuu 161202e199512 xx s 000 0 eng 10.1093/jxb/46.12.1915 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.12.1915 A simple photometric device analysing cuticular transport physiology: surfactant effect on permeability of isolated cuticular membranes of Prunus laurocerasus L [Elektronische Daten] [L. Schreiber, S. Bach, T. Kirsch, D. Knoll, K. Schalz, M. Riederer] 4-Nitrophenol permeabilities of astomatous cuticular membranes isolated from the upper surface of Prunus laurocerasus L. leaves were measured applying a newly developed photometric device. Isolated cuticles were mounted between donor and receiver compartments of a stainless steel transport chamber. 4-Nitrophenol was applied as non-dissociated species in citric buffer at pH 3.0 in the donor compartment and sampled as dissociated species in the receiver compartment in borate buffer at pH 9.0. Permeances, calculated from steady-state rates of 4-nitrophenol permeation, ranged from 1.73 ×10−10 m s−1 up to 38.4×10−10m s−1. They were in the same order of magnitude compared to published permeances obtained with a different method using radiolabelled 4-nitrophenol and isolated cuticles of Citrus aurantium L. In the presence of the surfactant Brij 30, which is a polydisperse alcohol ethoxylate, cuticular permeabilities increased on average by a factor of 37. Cuticles, initially having the lowest permeabilities, exhibited the highest increase of their permeabilities due to the surfactant and vice versa. This increase ofcuticular permeabilities in the presence of a surfactant is interpreted as a plasticizing effect of the surfactant molecules on the cuticular wax forming the cuticular transport barrier. Furthermore, surfactant-induced increases of cuticular permeabilities were reversible to a large extent. Permeabilities decreased again after the removal of Brij 30 reaching final values about 6-times higher compared to the initial permeabilities. This demonstrates that the surfactant and the permeating molecule must be present simultaneously in the cuticle in order to enhance cuticular permeation. Possible applications of this simple photometric device analysing further aspects of cuticular transport physiology are finally suggested. © Oxford University Press Methods Paper nationallicence Cuticular transport nationallicence leaf surface nationallicence permeability nationallicence plant cuticle nationallicence surfactant nationallicence Schreiber L. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut Bach S. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut Kirsch T. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut Knoll D. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut Schalz K. Fachbereich Biologie, Universität Kaiserslautern Erwin-Schrödinger-Straβe, D-67663 Kaiserslautern Germany aut Riederer M. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut Journal of Experimental Botany Oxford University Press 46/12(1995-12), 1915-1921 0022-0957 46:12<1915 1995 46 exbotj https://doi.org/10.1093/jxb/46.12.1915 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.12.1915 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schreiber L. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut NATIONALLICENCE 700 1- Bach S. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut NATIONALLICENCE 700 1- Kirsch T. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut NATIONALLICENCE 700 1- Knoll D. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut NATIONALLICENCE 700 1- Schalz K. Fachbereich Biologie, Universität Kaiserslautern Erwin-Schrödinger-Straβe, D-67663 Kaiserslautern Germany aut NATIONALLICENCE 700 1- Riederer M. Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik II, Universität Würzburg Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/12(1995-12), 1915-1921 0022-0957 46:12<1915 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford