caa a22 4500 397522762 CHVBK 20180308164637.0 cr unu---uuuuu 161202e199501 xx s 000 0 eng 10.1093/jxb/46.1.19 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.1.19 Localization of organelle DNA synthesis within the root apical meristem of rice [Elektronische Daten] [Takeshi Suzuki, Narie Sasaki, Atsushi Sakai, Shigeyuki Kawano, Tsuneyoshi Kuroiwa] DNA synthesis in cell nuclei and organelles in the root apical meristem of rice was analysed by anti-BrdU immunofluorescence microscopy to determine whether there is a specific order of these events in monocot roots. In the root meristem, organelle DNAs were synthesized in a specific region in the distal part of the root apical meristem, and were not synthesized in the root meristem‘s proximal region or the elongation zone. In contrast, cell nuclear DNA was synthesized throughout the root apical meristem, except in the quiescent centre. In the root cap of rice, DNA synthesis in both cell nuclei and organelle nucleoids was detected only in the two layers of cells at the proximal end, which is a striking characteristic of monocotyledonous plants. Moreover, to determine quantitatively the activity of DNA synthesis in cell nuclei and organelle nucleoids in micro-scale sections of plant tissues, we developed novel techniques for micro-scale hybridization and immuno-detection analysis. At the distal end of the root apical meristem, DNA levels of plastids and mitochondria were 4-fold and 5-fold greater than those in the elongation zone, respectively. Intracellular organelle DNA levels dropped rapidly as the distance from the root tip increased. The activity of organelle DNA synthesis in the distal end of the root apical meristem was about 10-fold greater than that in the elongation zone. Our present results confirm that nuclear and organelle DNA synthesis are not synchronized, but the latter occurs preferentially before multiple cell divisions. © Oxford University Press Research Papers nationallicence Organelle DNA synthesis nationallicence organelle nucleoids (organelle nuclei) nationallicence root apical meristem nationallicence anti-bromo-deoxyuridine immunofluorescence microscopy nationallicence rice nationallicence Suzuki Takeshi Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut Sasaki Narie Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut Sakai Atsushi Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut Kawano Shigeyuki Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut Kuroiwa Tsuneyoshi Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut Journal of Experimental Botany Oxford University Press 46/1(1995-01), 19-25 0022-0957 46:1<19 1995 46 exbotj https://doi.org/10.1093/jxb/46.1.19 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.1.19 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suzuki Takeshi Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut NATIONALLICENCE 700 1- Sasaki Narie Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut NATIONALLICENCE 700 1- Sakai Atsushi Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut NATIONALLICENCE 700 1- Kawano Shigeyuki Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut NATIONALLICENCE 700 1- Kuroiwa Tsuneyoshi Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/1(1995-01), 19-25 0022-0957 46:1<19 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford