caa a22 4500 397522789 CHVBK 20180308164637.0 cr unu---uuuuu 161202e199507 xx s 000 0 eng 10.1093/jxb/46.7.743 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.7.743 Levels of apoplastic solutes in developing strawberry fruit [Elektronische Daten] [Kirk W. Pomper, Patrick J. Breen] Solutes in the apoplast of sink organs can contributeto apoplastic water potential (ψaw) gradients that influence transport of water and assimilates. However, sampling the apoplast solution is often technically difficult. Three methods were used to collect apoplastic solution from developing strawberry fruit for determining apoplastic solute potential (ψas) and sucrose and glucose concentrations. In addition,ψas was was estimated from the difference between ψaw (measured via hygrometry) and fruit xylem pressure potential (ψx-press) (measured with a pressure chamber). As strawberry fruit developed, ψaw decreased linearly whereas ψx-press declined slightly then increased, therefore, the estimated ψaw was also decreased. Liquid was recovered from tissue plugs of ripening fruit using centrifugation.The solute potential (ψs) of these samples from pink and red fruit varied from -0.9 to -1.1 MPa and was similar to that of the bulk fruit solute potential (ψs-bulk).A novel method was developed to sample the apo-plastic solution, with little or no cell damage, by collecting liquid on filter paper discs inserted into the hollow cavity naturally formed in ripening fruit. Here the ψs was about -1.0 MPa, again similar to ψs-bulk, and sucrose and glucose concentrations were each near 50 mM. Solution recovered from ripening fruit by centrifugation and paper discs from the fruit cavity was red, suggesting leakage of anthocyanins into the apoplast. Liquid collected from the pedicel xylem of detached fruit using a pressure chamber was colour-less, had a high ψs (about -0.3 MPa), and low sugar concentrations (≺1mM) through out fruit development, suggesting that this method is not effective for sampling the fruit apoplast. Sugars accounted for onlyabout 35%of the ips of the apoplast of ripening strawberry fruit. © Oxford University Press Research Papers nationallicence Fruit development nationallicence solute potential nationallicence Fragaria x ananassa nationallicence Pomper Kirk W. Department of Horticulture, Agriculture and Life Sciences 4017, Oregon State University, Corvallis, OR 97331, USA aut Breen Patrick J. Department of Horticulture, Agriculture and Life Sciences 4017, Oregon State University, Corvallis, OR 97331, USA aut Journal of Experimental Botany Oxford University Press 46/7(1995-07), 743-752 0022-0957 46:7<743 1995 46 exbotj https://doi.org/10.1093/jxb/46.7.743 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.7.743 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pomper Kirk W. Department of Horticulture, Agriculture and Life Sciences 4017, Oregon State University, Corvallis, OR 97331, USA aut NATIONALLICENCE 700 1- Breen Patrick J. Department of Horticulture, Agriculture and Life Sciences 4017, Oregon State University, Corvallis, OR 97331, USA aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/7(1995-07), 743-752 0022-0957 46:7<743 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford