caa a22 4500 397522797 CHVBK 20180308164637.0 cr unu---uuuuu 161202e199507 xx s 000 0 eng 10.1093/jxb/46.7.767 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.7.767 A novel single-stranded DNA-specific endonuclease from pea chloroplasts [Elektronische Daten] [D. Kumar, S. Mukherjee, M.K. Reddy, K.K. Tewari] A nuclease with novel activities has been isolated and purified to apparent homogeneity from pea chloroplasts. The enzyme prefers single-stranded (ss) circular DNA; its activity being 1500-fold higher with the ss circular DNA than with the linear double-stranded DNA substrates. The single-stranded DNase activity is stable at moderately high temperature (50 °C) and inhibited in the presence of 75 mM NaCl. It binds negatively supercoiled DNA in the stoichiometric fashion, but behaves catalytically on the single-stranded circular DNA. Although the DNase activity does not recognize any specific nucleotide sequence, the co-operative mode of activity seems to be a novel one. The protein is a monomer of 35 kDa and binds with DNA predominantly through electrostatic interactions. The drug distamycin blocks the endonuclease activity suggesting that the protein binds at the minor groove of DNA. A RNase activity has also been found associated with the ss-DNA endonuclease. © Oxford University Press Research Papers nationallicence Pea nationallicence chloroplast nationallicence endonuclease nationallicence Kumar D. Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology NII Campus, New Delhi 110067, India aut Mukherjee S. Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology NII Campus, New Delhi 110067, India aut Reddy M.K. Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology NII Campus, New Delhi 110067, India aut Tewari K.K. Department of Molecular Biology and Biochemistry UCI, Irvine, CA, USA aut Journal of Experimental Botany Oxford University Press 46/7(1995-07), 767-776 0022-0957 46:7<767 1995 46 exbotj https://doi.org/10.1093/jxb/46.7.767 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.7.767 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumar D. Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology NII Campus, New Delhi 110067, India aut NATIONALLICENCE 700 1- Mukherjee S. Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology NII Campus, New Delhi 110067, India aut NATIONALLICENCE 700 1- Reddy M.K. Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology NII Campus, New Delhi 110067, India aut NATIONALLICENCE 700 1- Tewari K.K. Department of Molecular Biology and Biochemistry UCI, Irvine, CA, USA aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/7(1995-07), 767-776 0022-0957 46:7<767 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford