caa a22 4500 39754636X CHVBK 20180308164739.0 cr unu---uuuuu 161202e19960701xx s 000 0 eng 10.1093/aesa/89.4.576 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.4.576 Maternal Microinjection of the Endoparasitoid Cardiochiles diaphaniae (Hymenoptera: Braconidae) [Elektronische Daten] [James K. Presnail, Marjorie A. Hoy] Maternal microinjection is the injection of DNA into an adult female to deliver DNA to the eggs. Here we report the adaptation of this method to the parasitoid Cardiochiles diaphaniae (Hymenoptera: Braconidae). Results of preliminary dissections and injections with the plasmid pJKP2, followed by slot blot analysis, suggested that this method could be used to deliver injected DNA to the ovaries of this wasp. Subsequently, 16 adult females were injected and allowed to produce offspring (the G0 generation). Polymerase chain reaction analysis of the next (G1) generation revealed that several individuals contained the plasmid sequences. In a 2nd experiment, the plasmid phsopd, containing the parathion hydrolase gene (opd) of Pseudomonas diminuta, was injected. The plasmid probe hybridized to high molecular weight DNA from 3 of 38 G1 adults. A 4th adult produced a hybridization pattern consistent with integration of plasmid into the nuclear genome. The results indicate maternal microinjection can result in transformation of this parasitoid. © 1996 Entomological Society of America Genetics nationallicence Cardiochiles diaphaniae nationallicence maternal microinjection nationallicence Braconidae nationallicence transgenic nationallicence transformation nationallicence Presnail James K. Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611-0620. aut Hoy Marjorie A. Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611-0620. aut Annals of the Entomological Society of America Oxford University Press 89/4(1996-07-01), 576-580 0013-8746 89:4<576 1996 89 aesa https://doi.org/10.1093/aesa/89.4.576 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.4.576 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Presnail James K. Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611-0620 aut NATIONALLICENCE 700 1- Hoy Marjorie A. Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611-0620 aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/4(1996-07-01), 576-580 0013-8746 89:4<576 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford