caa a22 4500 397546521 CHVBK 20180308164739.0 cr unu---uuuuu 161202e19960301xx s 000 0 eng 10.1093/aesa/89.2.212 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.2.212 Reproductive Compatibility, Mating Behavior, and Random Amplified Polymorphic Dna Variability in Some Aphelinus asychis (Hymenoptera: Aphelinidae) Derived from the Old World [Elektronische Daten] [David J. Kazmer, Keira Maiden, Nathalie Ramualde, Dominique Coutinot, Keith R. Hopper] Reproductive compatibility, mating behavior, and random amplified polymorphic DNA (RAPID) variability were examined in samples of Aphelinus asychis obtained during foreign exploration for natural enemies of the Russian wheat aphid, Diuraphis noxia (Mordvilko). All specimens were reared initially from Diuraphis sp. collected at several Mediterranean basin localities and 2 localities in Central Asia. Using 7 laboratory cultures, we found 3 reciprocally and completely incompatible groups: the 1st group was represented by a culture from northcentral China, the 2nd group by a Kazakhstani culture, and a 3rd by 5 cultures from the Mediterranean basin (i.e., France, Greece, Morocco, Sicily, and Spain). One instance of partial, 1-way incompatibility was detected among the Mediterranean basin cultures. Behavioral experiments with 3 cultures showed that males respond to the sex pheromone of incompatible females and actively court them. However, females usually did not stop moving or elevate their abdomens in response to courtship by incompatible males. With 1 exception, genital contact and sperm transfer did not occur in incompatible pairings. Thus, rejection of incompatible males or failure to recognize incompatible males by females appeared to be 1 cause of incompatibility. In the exceptional case (Chinese males paired with Kazaklistani females), sperm transfer occurred but at significantly lower frequencies than in control pairings. Phenetic analysis of 61 RAPD loci in 6 laboratory cultures and 3 field samples from the Mediterranean basin delineated the same 3 groups as the compatibility experiment. Further work examining the geographic range, host range, and morphology of the incompatible groups is needed to clarify their taxonomic status. © 1996 Entomological Society of America Systematics nationallicence Aphelinus asychis nationallicence Diuraphis noxia nationallicence reproductive compatibility nationallicence random amplified polymorphic DNA nationallicence mating behavior nationallicence biological control nationallicence Kazmer David J. European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France. aut Maiden Keira European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France. aut Ramualde Nathalie European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France. aut Coutinot Dominique European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France. aut Hopper Keith R. European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France. aut Annals of the Entomological Society of America Oxford University Press 89/2(1996-03-01), 212-220 0013-8746 89:2<212 1996 89 aesa https://doi.org/10.1093/aesa/89.2.212 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.2.212 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kazmer David J. European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France aut NATIONALLICENCE 700 1- Maiden Keira European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France aut NATIONALLICENCE 700 1- Ramualde Nathalie European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France aut NATIONALLICENCE 700 1- Coutinot Dominique European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France aut NATIONALLICENCE 700 1- Hopper Keith R. European Biological Control Laboratory, USDA-ARS, BP 4168 Pare Agropolis II, 34092 Montpellier France aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/2(1996-03-01), 212-220 0013-8746 89:2<212 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford