caa a22 4500 397546688 CHVBK 20180308164740.0 cr unu---uuuuu 161202e19961101xx s 000 0 eng 10.1093/aesa/89.6.828 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.6.828 Biology of Trichogramma galloi and T. pretiosum (Hymenoptera: Trichogrammatidae) Reared in Vitro and in Vivo [Elektronische Daten] [Fernando LuíS Cônsoli, José Roberto Postali Parra] The life cycle and parasitization of Trichogramma galloi Zucchi and T. pretiosum Riley were studied on natural and factitious hosts, and on artificial diets. Eggs of sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Pyralidae), and tobacco budworm, Heliothis virescens (F.) (Lepidoptera: Noctuidae), were used as natural hosts of T. galloi and T. pretiosum, respectively. Eggs of Mediterranean flour moth, Anagasta kuehniella (Zeller) (Lepidoptera: Pyralidae), were used as factitious host for both parasitoids. T. galloi was reared in vitro on a diet of 70% hemolymph of larvae of corn earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), 20% chicken egg-yolk, 10% bovine fetal serum, and 0.2% streptomycin. T. pretiosum was reared on a similar diet, with 5% H. virescens egg juice replacing 5% bovine fetal serum. The egg-adult development of T. pretiosum reared on the artificial diet was delayed, whereas developmental differences were not found for T. galloi reared on natural or artificial diets. Survivorship was also reduced for both parasitoids reared on artificial diets. The parasitization and female longevity were reduced for parasitoids reared on the factitious host or artificial diets. No differences in adult size or in morphology of the genital apparatus were detected among parasitoids reared in vitro or in vivo; but wing and abdominal malformations occurred for parasitoids reared on artificial diets. © 1996 Entomological Society of America Arthropod Biology nationallicence Trichogramma galloi nationallicence Trichogramma pretiosum nationallicence artificial diet nationallicence biological control nationallicence parasitoids nationallicence in vitro rearing nationallicence Cônsoli Fernando LuíS Department of Entomology, Escola Superior de Agricultura "Luiz de Queiroz”—ESALQ/USP, CxP 9, 13418-900, Piracicaba, São Paulo, Brazil. aut Parra José Roberto Postali Department of Entomology, Escola Superior de Agricultura "Luiz de Queiroz”—ESALQ/USP, CxP 9, 13418-900, Piracicaba, São Paulo, Brazil. aut Annals of the Entomological Society of America Oxford University Press 89/6(1996-11-01), 828-834 0013-8746 89:6<828 1996 89 aesa https://doi.org/10.1093/aesa/89.6.828 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.6.828 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cônsoli Fernando LuíS Department of Entomology, Escola Superior de Agricultura "Luiz de Queiroz”—ESALQ/USP, CxP 9, 13418-900, Piracicaba, São Paulo, Brazil aut NATIONALLICENCE 700 1- Parra José Roberto Postali Department of Entomology, Escola Superior de Agricultura "Luiz de Queiroz”—ESALQ/USP, CxP 9, 13418-900, Piracicaba, São Paulo, Brazil aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/6(1996-11-01), 828-834 0013-8746 89:6<828 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford