caa a22 4500 397546726 CHVBK 20180308164740.0 cr unu---uuuuu 161202e19961101xx s 000 0 eng 10.1093/aesa/89.6.788 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.6.788 18S Ribosomal Rna Genes of Insects: Primary Structure of the Genes and Molecular Phylogeny of the Holometabola [Elektronische Daten] [Nicolas Chalwatzis, Jörg Hauf, Yves Van De Peer, Ragnar Kinzelbach, Friedrich K. Zimmermann] The 18S ribosomal RNA genes of 19 insect species, including 12 Holometabola, were cloned and sequenced. The genes of the insect species investigated so far vary in length from 1,809 to 3,316 bp. The genes were aligned according to the secondary structure model of the 18S ribosomal RNA. An average of 1,580 aligned nucleotide positions per gene was used for the calculation of phylogenetic trees with sequences of this and previous studies. Neighbor-joining trees were calculated with gamma, substitution rate calibration, and LogDet distances. Informative alignment positions were used for a maximum parsimony analysis. The robustness of the trees was tested by bootstrapping and by branch support calculations. All the major groups of the Holometabola, which are commonly regarded as monophyletic except for the Mecoptera, were supported with the neighbor-joining analysis. Some of these groups were not represented in the most parsimonious trees or had a bootstrap support of <80%. However, a sister group relationship of the Strepsiptera and the Diptera was found with both methods and with corrections for different substitution rates at different sites and for differences in the nucleotide composition. © 1996 Entomological Society of America Systematics nationallicence 18S rRNA genes nationallicence Strepsiptera nationallicence Mecoptera nationallicence Siphonaptera nationallicence Holometabola nationallicence phylogeny nationallicence Chalwatzis Nicolas Technische Hochschule Darmstadt, Institut für Zoologie, Schnittspahnstr. 3, D-64287 Darmstadt, Germany. aut Hauf Jörg Technische Hochschule Darmstadt, Institut für Mikrobiologie, Schnittspahnstr.10, D-64287 Darmstadt, Germany. aut Van De Peer Yves Department Biochemie, Universiteit Anrwerpen (UIA), Universiteitsplein 1, B-2610 Antwerp, Belgium. aut Kinzelbach Ragnar Technische Hochschule Darmstadt, Institut für Zoologie, Schnittspahnstr. 3, D-64287 Darmstadt, Germany. aut Zimmermann Friedrich K. Technische Hochschule Darmstadt, Institut für Mikrobiologie, Schnittspahnstr.10, D-64287 Darmstadt, Germany. aut Annals of the Entomological Society of America Oxford University Press 89/6(1996-11-01), 788-803 0013-8746 89:6<788 1996 89 aesa https://doi.org/10.1093/aesa/89.6.788 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.6.788 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chalwatzis Nicolas Technische Hochschule Darmstadt, Institut für Zoologie, Schnittspahnstr. 3, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Hauf Jörg Technische Hochschule Darmstadt, Institut für Mikrobiologie, Schnittspahnstr.10, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Van De Peer Yves Department Biochemie, Universiteit Anrwerpen (UIA), Universiteitsplein 1, B-2610 Antwerp, Belgium aut NATIONALLICENCE 700 1- Kinzelbach Ragnar Technische Hochschule Darmstadt, Institut für Zoologie, Schnittspahnstr. 3, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Zimmermann Friedrich K. Technische Hochschule Darmstadt, Institut für Mikrobiologie, Schnittspahnstr.10, D-64287 Darmstadt, Germany aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/6(1996-11-01), 788-803 0013-8746 89:6<788 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford