caa a22 4500 397546750 CHVBK 20180308164740.0 cr unu---uuuuu 161202e19961101xx s 000 0 eng 10.1093/aesa/89.6.804 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.6.804 Molecular Differentiation of Alfalfa Weevil Strains (Coleoptera: Curculionidae) [Elektronische Daten] [S. J. Erney, K. P. Pruess, S. D. Danielson, T. O. Powers] Mitochondrial DNA was amplified and sequenced from eastern, western, and Egyptian strains of alfalfa weevil, Hypera postica (Gyllenhal). Eastern and Egyptian weevils differed at only 2 nucleotide sites in 1,031 base pairs sequenced; western weevils differed by 5% sequence divergence. Three restriction sites were identified which separated eastern and western haplotypes. No intrastrain polymorphism was detected in 150 weevils from Nebraska. Collections from Lincoln in eastern Nebraska and Scottsbluff in western Nebraska were fixed for the eastern and western haplotypes, respectively. Eastern and western haplotypes were found together in the same fields in a broad overlap region in central Nebraska. © 1996 Entomological Society of America Systematics nationallicence Hypera postica nationallicence alfalfa weevil strains nationallicence mtDNA nationallicence PCR-RFLP nationallicence DNA sequence nationallicence Erney S. J. aut Pruess K. P. aut Danielson S. D. aut Powers T. O. Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583-0722. aut Annals of the Entomological Society of America Oxford University Press 89/6(1996-11-01), 804-811 0013-8746 89:6<804 1996 89 aesa https://doi.org/10.1093/aesa/89.6.804 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.6.804 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Erney S. J. aut NATIONALLICENCE 700 1- Pruess K. P. aut NATIONALLICENCE 700 1- Danielson S. D. aut NATIONALLICENCE 700 1- Powers T. O. Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583-0722 aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/6(1996-11-01), 804-811 0013-8746 89:6<804 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford