caa a22 4500 397546890 CHVBK 20180308164740.0 cr unu---uuuuu 161202e19960901xx s 000 0 eng 10.1093/aesa/89.5.728 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.5.728 Molecular Population Genetics of Sodium Channel and Juvenile Hormone Esterase Markers in Relation to Pyrethroid Resistance in Heliothis virescens (Lepidoptera: Noctuidae) [Elektronische Daten] [Martin F. J. Taylor, Yoonseong Park, Ying Shen] Pyrethroid insecticide resistance in strains of tobacco budworm, Heliothis virescens (F.), has been linked to a locus hscp, which encodes the target of action of pyrethroids, a voltage-gated sodium channel (α subunit). Using denaturing gradient gel electrophoresis, we examined allelic variation for the DNA marker locus Hpy within this gene, and for an additional marker locus Hejs within a juvenile hormone esterase gene, for population samples from the southern United States in 1990 and 1995. FST, the estimate of genetic variance among populations for Hpy was 0.041 (SE = 0.005), significantly greater than the FST of 0.001 (SE = 0.003) for Hejs, and other estimates reported for allozyme loci in a 1989 collection over a similar geographic range. The greater variance for Hpy suggests that selection had been acting at or near the hscp locus. There was a significant excess of Hpyl, 3, and 5 homozygotes in the 1990 collection, relative to Hardy-Weinberg expectation. However, there was also excess homozygosity for Hejs alleles, for reasons unknown. Hpy3, the 3rd most common allele of Hpy, significantly increased after pyrethroid selection on Texas and Louisiana samples of 1990, and on progeny of a Louisiana sample of 1995. This indicates positive linkage disequilibrium between Hpy3 and presumed resistance-conferring allele(s) at another locus. Disequilibrium has persisted from 1990 to 1995. We conclude that one or more mutations elsewhere in the hscp gene confer resistance to pyrethroids in populations of H. virescens. © 1996 Entomological Society of America Genetics nationallicence Heliothis virescens nationallicence pyrethroid resistance nationallicence population genetics nationallicence sodium channel nationallicence juvenile hormone esterase nationallicence Taylor Martin F. J. Department of Entomology and Center for Insect Science, University of Arizona, Tucson, AZ 85721. aut Park Yoonseong Department of Entomology and Center for Insect Science, University of Arizona, Tucson, AZ 85721. aut Shen Ying Department of Entomology and Center for Insect Science, University of Arizona, Tucson, AZ 85721. aut Annals of the Entomological Society of America Oxford University Press 89/5(1996-09-01), 728-738 0013-8746 89:5<728 1996 89 aesa https://doi.org/10.1093/aesa/89.5.728 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.5.728 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taylor Martin F. J. Department of Entomology and Center for Insect Science, University of Arizona, Tucson, AZ 85721 aut NATIONALLICENCE 700 1- Park Yoonseong Department of Entomology and Center for Insect Science, University of Arizona, Tucson, AZ 85721 aut NATIONALLICENCE 700 1- Shen Ying Department of Entomology and Center for Insect Science, University of Arizona, Tucson, AZ 85721 aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/5(1996-09-01), 728-738 0013-8746 89:5<728 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford