caa a22 4500 397546920 CHVBK 20180308164740.0 cr unu---uuuuu 161202e19960901xx s 000 0 eng 10.1093/aesa/89.5.645 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.5.645 Molecular and Morphological Characters Discriminate Sitophilus oryzae and S. zeamais (Coleoptera: Curculionidae) and Confirm Reproductive Isolation [Elektronische Daten] [Purnama Hidayat, Thomas W. Phillips, Richard H. Ffrench-Constant] The Rice Weevil, Sitophilus oryzae (L.), and the maize weevil, S. zeamais Motschulsky, are sibling species of grain weevils that are usually distinguished by grain preferences and subtle differences in morphology. Previous findings of successful laboratory hybridization, genetic similarity of allozymes and chromosomes, and identity of aggregation pheromones raised questions about the validity of S. oryzae and S. zeamais as reproductively isolated biological species. We used molecular techniques to test the hypothesis that individuals assigned as S. oryzae or S. zeamais by morphological criteria represent members of 2 distinct gene pools, and hence are reproductively isolated species. Weevils from 18 different localities, which were collected from Africa, Australia, Asia, the south Pacific, and North America, were studied. All individuals were scored for the presence or absence of morphological characters that have been used historically for species determination. In most cases, determinations for the same individual were not consistent, depending on the morphological character used. The polymerase chain reaction (PCR) was used on the same specimens to analyze randomly amplified polymorphic DNA (RAPD-PCR) markers and to amplify selectively regions of mitochondrial DNA for analysis of restriction site polymorphisms with restriction endonucleases (RFLP-PCR). Both methods yielded markers that were consistently associated with either presence or absence of specific genitalic characters in both males and females. This correlation of molecular markers with genitalic morphotypes was consistent in all specimens studied, whether collected sympatrically from the same farms or from widely separated geographic populations, and supports a model of 2 reproductively isolated species. Other morphological characters involving pronotal punctures proved unreliable as correlates with genetic markers and are not useful for species diagnosis. © 1996 Entomological Society of America Systematics nationallicence stored products nationallicence grain nationallicence mitochondrial DNA nationallicence polymerase chain reaction nationallicence Hidayat Purnama Department of Entomology, University of Wisconsin, Madison, WI 53706. aut Phillips Thomas W. Stored-Product Incest Research Unit, USDA-ARS, Department of Entomology, University of Wisconsin, Madison, WI 53706. aut Ffrench-Constant Richard H. Department of Entomology, University of Wisconsin, Madison, WI 53706. aut Annals of the Entomological Society of America Oxford University Press 89/5(1996-09-01), 645-652 0013-8746 89:5<645 1996 89 aesa https://doi.org/10.1093/aesa/89.5.645 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.5.645 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hidayat Purnama Department of Entomology, University of Wisconsin, Madison, WI 53706 aut NATIONALLICENCE 700 1- Phillips Thomas W. Stored-Product Incest Research Unit, USDA-ARS, Department of Entomology, University of Wisconsin, Madison, WI 53706 aut NATIONALLICENCE 700 1- Ffrench-Constant Richard H. Department of Entomology, University of Wisconsin, Madison, WI 53706 aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/5(1996-09-01), 645-652 0013-8746 89:5<645 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford