caa a22 4500 397546939 CHVBK 20180308164740.0 cr unu---uuuuu 161202e19960901xx s 000 0 eng 10.1093/aesa/89.5.694 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.5.694 Mycetocyte Inclusion in the Oocytes of Bemisia argentifolii (Homoptera: Aleyrodidae) [Elektronische Daten] [Heather S. Costa, Nick C. Toscano, Thomas J. Henneberry] This study investigates the process of transfer of endosymbiotic organisms from adult silverleaf whitefly, Bemisia argentifolii Bellows & Perring (Bellows et al. 1994) (also known as Bemisia tabaci B biotype), into the developing ova. In dissected females, individual mycetocyte cells containing microorganisms were scattered singly among developing oocytes. Oocytes with mycetocytes included were first observed in females 16 h after emergence. The mean number of oocytes >0.1 mm in length per female increased through the 4th d after adult eclosion then leveled off. The number of oocytes containing mycetocytes followed a similar pattern. Stages of mycetocyte inclusion followed a pattern based on the size of the oocyte. Oocytes became associated with a single mycetocyte cell when they were a mean of length of 0.135 ± 0.003 mm (minimum of 0.11 mm). All mature oocytes examined contained a mycetocyte. Mycetocytes were observed inside a common membrane with oocytes, at what becomes the pedicel end of the ova, when oocytes were an average of 0.147 ± 0.004 mm long. In the final stages of ovum development, the plasma of the oocyte completely surrounded the mycetocyte and the chorion was thickened. Mycetocytes included in oocytes had a mean length of 33 ± 0.4 µm and width of 27 ± 0.5 µm. Although the females that were dissected had the opportunity to oviposit, some retained several fully developed ova. Because inclusion of mycetocytes into oocytes is a continuous process, manipulations of endosymbionts before inclusion into the oocytes should be possible at any time during adult life; however, earlier treatment of an individual would probably affect a greater proportion of their offspring. © 1996 Entomological Society of America Arthropod Biology nationallicence Bemisia argentifolii nationallicence oocyte nationallicence whitefly nationallicence endosymbionts nationallicence Costa Heather S. Department of Entomology, University of California, Riverside, CA 92521. aut Toscano Nick C. Department of Entomology, University of California, Riverside, CA 92521. aut Henneberry Thomas J. Western Cotton Research Laboratory, USDA-ARS, 4135 East Broadway, Phoenix, AZ 85040. aut Annals of the Entomological Society of America Oxford University Press 89/5(1996-09-01), 694-699 0013-8746 89:5<694 1996 89 aesa https://doi.org/10.1093/aesa/89.5.694 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.5.694 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Costa Heather S. Department of Entomology, University of California, Riverside, CA 92521 aut NATIONALLICENCE 700 1- Toscano Nick C. Department of Entomology, University of California, Riverside, CA 92521 aut NATIONALLICENCE 700 1- Henneberry Thomas J. Western Cotton Research Laboratory, USDA-ARS, 4135 East Broadway, Phoenix, AZ 85040 aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/5(1996-09-01), 694-699 0013-8746 89:5<694 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford