caa a22 4500 397547226 CHVBK 20180308164741.0 cr unu---uuuuu 161202e19960501xx s 000 0 eng 10.1093/aesa/89.3.420 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.3.420 Infectious Enterococcus from Heliothis virescens × H. subflexa Backcross Hybrids (Lepidoptera: Noctuidae) [Elektronische Daten] [S. G. Miller, R. D. Miller] Plating assays have revealed the presence of a bacterium in extracts of tissues from backcross hybrid insects, derived from crosses between Heliothis virescens and H. subflexa, a bacterium found rarely and in much lower abundance in either of the 2 parent species. The bacterium is primarily localized within the gut but is also consistently recovered in small numbers from other somatic and germinal tissues. Fatty acid profile analyses and the sequence of its 16s ribosomal RNA indicate that the bacterium is an Enterococcus related to Enterococcus sulfureus and E. casseliflavus, species that previously have been encountered in insects and on plant tissues. Injection of the Enterococcus into H. virescens hosts results in the establishment of a bacterial infection similar to that of naturally infected backcross hybrids in terms of tissue distribution and relative magnitude among tissues. Like backcross hybrid males, almost 90% of H. virescens male moths infected as larvae were sterile in single pair test matdngs. The inability to either rescue male fertility in backcross lines cured of the Enterococcus or to observe characteristic abnormalities in sperm ultrastructure, however, casts doubt on the likelihood that this microorganism is the proximate cause of male sterility in backcross hybrid insects. © 1996 Entomological Society of America Genetics nationallicence Heliothis virescens nationallicence Heliothis subflexa nationallicence backcross hybrids nationallicence Enterococcus nationallicence male sterility nationallicence Miller S. G. Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA-ARS, Gainesville, FL 32608. aut Miller R. D. Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA-ARS, Gainesville, FL 32608. aut Annals of the Entomological Society of America Oxford University Press 89/3(1996-05-01), 420-427 0013-8746 89:3<420 1996 89 aesa https://doi.org/10.1093/aesa/89.3.420 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.3.420 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miller S. G. Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA-ARS, Gainesville, FL 32608 aut NATIONALLICENCE 700 1- Miller R. D. Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA-ARS, Gainesville, FL 32608 aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/3(1996-05-01), 420-427 0013-8746 89:3<420 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford