caa a22 4500 397547552 CHVBK 20180308164742.0 cr unu---uuuuu 161202e19960101xx s 000 0 eng 10.1093/aesa/89.1.122 doi (NATIONALLICENCE)oxford-10.1093/aesa/89.1.122 Inheritance of Random Amplified Polymorphic DNA Markers in Anastrepha suspensa (Diptera: Tephritidae) [Elektronische Daten] [Raymond J. Schnell, Paul M. Madeira, Michael K. Hennessey, Jennifer L. Sharp] Two full-sibling families of the Caribbean fruit fly, Anastrepha suspensa (Loew), were produced by controlled mating from the USDA-ARS colonies located at Miami, FL. One family was produced from a colony population reared on a corncob diet as larvae. The other family was produced from a colony population reared on an agar diet as larvae. Colonies originated from a common source but each had been maintained as an isolated population for >800 generations. DNA was extracted from both parents and 36 full-sibling progeny from both families. Sixty-three random amplified polymorphic DNA (RAPD) primers were screened using DNA from the 4 parental flies and 97% (61) produced amplification products. Six polymorphic primers were selected for the analysis. These 6 primers amplified 127. bands in both the corncob and the agar flies; of these, 87 and 77 bands were analyzed in the corncob and agar flies, respectively. In the corncob flies, 55.2% of the analyzed loci were polymorphic: 49.4% were polymorphic in the agar flies. Both 1:1 and 3:1 segregation ratios of polymorphic loci were observed with 24 and 28 loci segregating 1:1, and 13 and 9 loci segregating 3:1 in the corncob and agar flies, respectively. Most RAPD primers generated polymorphic bands that segregated as expected. This indicated that polymorphic RAPD bands are useful as genetic markers in Caribbean fruit fly. The markers are potentially useful for host and geographic population studies as they relate to quarantine issues. © 1996 Entomological Society of America Genetics nationallicence Caribbean fruit fly nationallicence DNA nationallicence Insecta nationallicence population genetics nationallicence quarantine nationallicence random amplified polymorphic-polymerase chain reaction nationallicence Schnell Raymond J. Subtropical Horticulture Research Station, USDA-ARS, 13601 Old Cutler Road, Miami, FL 33158. aut Madeira Paul M. Subtropical Horticulture Research Station, USDA-ARS, 13601 Old Cutler Road, Miami, FL 33158. aut Hennessey Michael K. Subtropical Horticulture Research Station, USDA-ARS, 13601 Old Cutler Road, Miami, FL 33158. aut Sharp Jennifer L. Subtropical Horticulture Research Station, USDA-ARS, 13601 Old Cutler Road, Miami, FL 33158. aut Annals of the Entomological Society of America Oxford University Press 89/1(1996-01-01), 122-128 0013-8746 89:1<122 1996 89 aesa https://doi.org/10.1093/aesa/89.1.122 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/aesa/89.1.122 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schnell Raymond J. Subtropical Horticulture Research Station, USDA-ARS, 13601 Old Cutler Road, Miami, FL 33158 aut NATIONALLICENCE 700 1- Madeira Paul M. Subtropical Horticulture Research Station, USDA-ARS, 13601 Old Cutler Road, Miami, FL 33158 aut NATIONALLICENCE 700 1- Hennessey Michael K. Subtropical Horticulture Research Station, USDA-ARS, 13601 Old Cutler Road, Miami, FL 33158 aut NATIONALLICENCE 700 1- Sharp Jennifer L. Subtropical Horticulture Research Station, USDA-ARS, 13601 Old Cutler Road, Miami, FL 33158 aut NATIONALLICENCE 773 0- Annals of the Entomological Society of America Oxford University Press 89/1(1996-01-01), 122-128 0013-8746 89:1<122 1996 89 aesa Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford