caa a22 4500 397552254 CHVBK 20180308164753.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00127.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00127.x PCR-RFLP and total DNA homology revealed three related genomic species among broad-host-range Frankia strains [Elektronische Daten] [Erica Lumini, Marco Bosco, Maria P. Fernandez] Restriction fragment length polymorphism (RFLP) analysis of the PCR amplified nifD-K intergenic spacer (IGS) region was used to cluster 22 Frankia strains of the Elaeagnus host specificity group into seven genomic groups and to measure the degree of genetic similarity among them. This PCR-RFLP analysis could assign freshly isolated strains to described genomic species and revealed genomic groups not yet described among Frankia strains of the Elaeagnus specificity group. Six broad-host-range Frankia strains, infective on both Alnus and Elaeagnus, fell into three closely related PCR-RFLP clusters. DNA-DNA hybridization was then used to establish the correlations between PCR-RFLP clusters and total DNA relatedness groups. The three PCR-RFLP clusters agreed with two new and one reference genomic species, indicating that Frankia ability to nodulate with Alnus and Elaeagnus is a monophyletic trait shared by three genomic species. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Frankia nationallicence Elaeagnus nationallicence PCR-RFLP patterns nationallicence DNA-DNA hybridization nationallicence Genomic species nationallicence Lumini Erica Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Microbiologia Applicata, Università degli Studi di Firenze, Piazzale delle Cascine 27, 50144 Florence, Italy aut Bosco Marco Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Microbiologia Applicata, Università degli Studi di Firenze, Piazzale delle Cascine 27, 50144 Florence, Italy aut Fernandez Maria P. Ecologie Microbienne du Sol, UMR CNRS 5557, Bât. 741, Université Claude Bernard Lyon 1, 43 Bd. du 11 Novembre 1918, 69622 Villeurbanne Cedex, France aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/4(1996-12), 303-311 21:4<303 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00127.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00127.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lumini Erica Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Microbiologia Applicata, Università degli Studi di Firenze, Piazzale delle Cascine 27, 50144 Florence, Italy aut NATIONALLICENCE 700 1- Bosco Marco Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Microbiologia Applicata, Università degli Studi di Firenze, Piazzale delle Cascine 27, 50144 Florence, Italy aut NATIONALLICENCE 700 1- Fernandez Maria P. Ecologie Microbienne du Sol, UMR CNRS 5557, Bât. 741, Université Claude Bernard Lyon 1, 43 Bd. du 11 Novembre 1918, 69622 Villeurbanne Cedex, France aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/4(1996-12), 303-311 21:4<303 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford