caa a22 4500 397552297 CHVBK 20200225061703.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00128.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00128.x Marxsen Jürgen Limnologische Flußstation des Max-Planck-Instituts für Limnologie, P.O. Box 260, D-36105 Schlitz, Germany Measurement of bacterial production in stream-bed sediments via leucine incorporation [Elektronische Daten] [Jürgen Marxsen] The leucine incorporation technique was evaluated and optimised for measuring bacterial production in stream-bed sediments. The original procedure was modified in order to obtain reliable production estimates in this habitat. This included the use of higher leucine concentrations (50 μmol l−1) for obtaining substrate saturation, and an enhanced protein extraction procedure after sample fixation. The leucine method was combined with a perfused core technique. Water containing 14C-labelled leucine was perfused up through sediment cores, enabling the measurement of bacterial production in an experimental situation resembling natural conditions in the stream bed. Bacterial production in the Breitenbach, a small upland stream in Central Germany, showed a high degree of spatial variability in the sandy stream bed. It was related closely to sediment organic matter content, whereas varying perfusion rates had less influence. Annual bacterial production was estimated at about 200 g C m−2, demonstrating the potential for bacteria to act as a food resource for benthic fauna in this stream. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Stream nationallicence Sediment nationallicence Bacterial production nationallicence Leucine incorporation nationallicence Sediment core perfusion technique nationallicence FEMS Microbiology Ecology Blackwell Publishing Ltd 21/4(1996-12), 313-325 21:4<313 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00128.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00128.x text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Marxsen Jürgen Limnologische Flußstation des Max-Planck-Instituts für Limnologie, P.O. Box 260, D-36105 Schlitz, Germany NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/4(1996-12), 313-325 21:4<313 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence SWISSBIB 397552297 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford