caa a22 4500 397552319 CHVBK 20180308164753.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00121.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00121.x The role of polyglucose in oxygen-dependent respiration by a new strain of Desulfovibrio salexigens [Elektronische Daten] [Ed W.J. van Niel, Teresa M. Pedro Gomes, Anne Willems, Matthew D. Collins, Rudolf A. Prins, Jan C. Gottschal] Desulfovibrio salexigens strain Mastl was isolated from the oxic/anoxic interface of a marine sediment. Growth under sulfate-reducing conditions was accompanied by polyglucose accumulation in the cell with every substrate tested. Highest polyglucose storage was found with glucose (0.8-1.0 g polyglucose (g protein)−1), but the growth rate with this substrate was very low (0.015 h−1). Anaerobically grown cells of strain Mastl exhibited immediate oxygen-dependent respiration. The endogenous oxygen reduction rate was proportional to the polyglucose content. The rate of aerobic respiration of pyruvate was also directly related to the polyglucose content indicating that this organism was only able to respire with oxygen as long as polyglucose was present. Maximum oxygen reduction rates were found at air saturating concentrations and were relatively low (3-50 nmol O2 min−1 (mg protein)−1). Catalase was constitutively present in anaerobically grown cells. When batch cultures were exposed to oxygen, growth ceased immediately and polyglucose was oxidized to acetate within 40-50 h. Like the oxygen reduction activity, the nitro blue tetrazolium (NBT)-reduction activity in these cells was proportional to the polyglucose content. Under anaerobic starvation conditions there was no correlation between the NBT-reduction activity and polyglucose concentration and polyglucose was degraded slowly within 240 h. The ecological significance of aerobic polyglucose consumption is discussed. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Desulfovibrio salexigens nationallicence Sulfate reduction nationallicence Glucose nationallicence Polyglucose nationallicence Oxygen-dependent respiration nationallicence van Niel Ed W.J. Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands aut Pedro Gomes Teresa M. Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands aut Willems Anne Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ, UK aut Collins Matthew D. Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ, UK aut Prins Rudolf A. Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands aut Gottschal Jan C. Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/4(1996-12), 243-253 21:4<243 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00121.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00121.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Niel Ed W.J. Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands aut NATIONALLICENCE 700 1- Pedro Gomes Teresa M. Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands aut NATIONALLICENCE 700 1- Willems Anne Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ, UK aut NATIONALLICENCE 700 1- Collins Matthew D. Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ, UK aut NATIONALLICENCE 700 1- Prins Rudolf A. Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands aut NATIONALLICENCE 700 1- Gottschal Jan C. Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/4(1996-12), 243-253 21:4<243 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford