caa a22 4500 397552424 CHVBK 20180308164753.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00313.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00313.x Study of nonculturable Legionella pneumophila cells during multiple-nutrient starvation [Elektronische Daten] [Hiroyuki Yamamoto, Yasuhiro Hashimoto, Takayuki Ezaki] The nonculturable form of Legionella pneumophila in multiple-nutrient starvation culture was studied. During extended starvation, the total direct counts (TDC) of L. pneumophila did not change significantly, but no colonies were detected on day 50. Quantitative PCR detection of L. pneumophila DNA demonstrated that nonculturable cells retained PCR-detectable DNA even after starvation for 300 days, and part of the nonculturable population possessed nonspecific esterase activity. However, resuscitation trials of nonculturable L. pneumophila on day 100 of starvation recovered no colony-forming units, and electrophoresis of nucleic acids extracted from nonculturable cells revealed rRNA subunit (23S, 16S and 5S) degradation. When legionellae have lost the ability to multiply, at least some DNA and enzyme functions may be retained for prolonged periods. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Starvation-survival nationallicence Viability nationallicence Nonculturable form nationallicence Legionella pneumophila nationallicence PCR nationallicence Yamamoto Hiroyuki Department of Microbiology, Gifu University School of Medicine, Tsukasa-Machi 40, Gifu 500, Japan aut Hashimoto Yasuhiro Department of Microbiology, Gifu University School of Medicine, Tsukasa-Machi 40, Gifu 500, Japan aut Ezaki Takayuki Department of Microbiology, Gifu University School of Medicine, Tsukasa-Machi 40, Gifu 500, Japan aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/3(1996-07), 149-154 20:3<149 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00313.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00313.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamamoto Hiroyuki Department of Microbiology, Gifu University School of Medicine, Tsukasa-Machi 40, Gifu 500, Japan aut NATIONALLICENCE 700 1- Hashimoto Yasuhiro Department of Microbiology, Gifu University School of Medicine, Tsukasa-Machi 40, Gifu 500, Japan aut NATIONALLICENCE 700 1- Ezaki Takayuki Department of Microbiology, Gifu University School of Medicine, Tsukasa-Machi 40, Gifu 500, Japan aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/3(1996-07), 149-154 20:3<149 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford