caa a22 4500 397552459 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00314.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00314.x Survival of potentially pathogenic human-associated bacteria in the rhizosphere of hydroponically grown wheat [Elektronische Daten] [Anabelle Morales, Jay L. Garland, Daniel V. Lim] Plants may serve as reservoirs for human-associated bacteria (H-AB) in long-term space missions containing bioregenerative life support systems. The current study examined the abilities of five human-associated potential pathogens, Pseudomonas aeruginosa, Pseudomonas cepacia, Staphylococcus aureus, Streptococcus pyogenes and Escherichia coli, to colonize and grow in the rhizosphere of hydroponically grown wheat, a candidate crop for life support. All of these bacteria have been recovered from past NASA missions and present potential problems for future missions. The abilities of these organisms to adhere to the roots of axenic five-day-old wheat (Triticum aestivum L. cv. Yecora rojo) were evaluated by enumeration of the attached organisms after a one hour incubation of roots in a suspension (approximately 108 cfu ml−1) of the H-AB. Results showed that a greater percentage of P. aeruginosa cells adhered to the wheat roots than the other four H-AB. Similarly incubated seedlings were also grown under attempted axenic conditions for seven days to examine the potential of each organism to proliferate in the rhizosphere (root colonization capacity). P. cepacia and P. aeruginosa showed considerable growth, E. coli and S. aureus showed no significant growth, and S. pyogenes died off in the wheat rhizosphere. Studies examining the effects of competition on the survival of these microorganisms indicated that P. aeruginosa was the only organism that survived in the rhizosphere of hydroponically grown wheat in the presence of different levels of microbial competition. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Human-associated bacteria (H-AB) nationallicence Rhizosphere nationallicence Adherence nationallicence Root colonization capacity nationallicence Microbial competition nationallicence Morales Anabelle Department of Biology, LIF 136, University of South Florida, Tampa, FL 33620, USA aut Garland Jay L. Dynamac Corporation, Mail Code: DYN-3, Kennedy Space Center, FL 32899, USA aut Lim Daniel V. Department of Biology, LIF 136, University of South Florida, Tampa, FL 33620, USA aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/3(1996-07), 155-162 20:3<155 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00314.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00314.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morales Anabelle Department of Biology, LIF 136, University of South Florida, Tampa, FL 33620, USA aut NATIONALLICENCE 700 1- Garland Jay L. Dynamac Corporation, Mail Code: DYN-3, Kennedy Space Center, FL 32899, USA aut NATIONALLICENCE 700 1- Lim Daniel V. Department of Biology, LIF 136, University of South Florida, Tampa, FL 33620, USA aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/3(1996-07), 155-162 20:3<155 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford