caa a22 4500 397552491 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00322.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00322.x A Benthic Gradient Chamber for culturing phototrophic sulfur bacteria on reconstituted sediments [Elektronische Daten] [Olivier Pringault, Rutger de Wit, Pierre Caumette] The growth of phototrophic sulfur bacteria in benthic systems is restricted to well-defined layers within the sedimentary oxygen, sulfide, pH and light gradients. In order to culture these microorganisms under more ecologically relevant conditions, we have developed a Benthic Gradient Chamber (BGC) in which phototrophic sulfur bacteria can be grown within experimentally imposed solute and light gradients. The new autoclavable device is composed of a reconstituted sand core sandwiched in between a lower anoxic sulfide-containing compartment and an upper oxic compartment. The core can be illuminated from above by a collimated light beam. An axenic biofilm of Thiocapsa roseopersicina strain EP 2204 developed from a tiny inoculum within the sand core, using a 5-week incubation period and a 16:8 h light/dark illumination regime. The metabolic activities in this biofilm were inferred from the analyses of oxygen, sulfide and pH profiles, and their shifts during light-dark cycles. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Thiocapsa roseopersicina nationallicence Oxygen nationallicence Sulfide gradient nationallicence Light gradient nationallicence Axenic biofilm nationallicence Diffusion-limited growth nationallicence Sulfide oxidation nationallicence Minielectrode nationallicence Pringault Olivier Laboratoire d'Océanographie Biologique, Université de Bordeaux I, C.N.R.S.-U.R.A. 197, 2, rue du Prof. Jolyet, F-33120 Arcachon, France aut de Wit Rutger Laboratoire d'Océanographie Biologique, Université de Bordeaux I, C.N.R.S.-U.R.A. 197, 2, rue du Prof. Jolyet, F-33120 Arcachon, France aut Caumette Pierre Laboratoire d'Océanographie Biologique, Université de Bordeaux I, C.N.R.S.-U.R.A. 197, 2, rue du Prof. Jolyet, F-33120 Arcachon, France aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/4(1996-08), 237-250 20:4<237 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00322.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00322.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pringault Olivier Laboratoire d'Océanographie Biologique, Université de Bordeaux I, C.N.R.S.-U.R.A. 197, 2, rue du Prof. Jolyet, F-33120 Arcachon, France aut NATIONALLICENCE 700 1- de Wit Rutger Laboratoire d'Océanographie Biologique, Université de Bordeaux I, C.N.R.S.-U.R.A. 197, 2, rue du Prof. Jolyet, F-33120 Arcachon, France aut NATIONALLICENCE 700 1- Caumette Pierre Laboratoire d'Océanographie Biologique, Université de Bordeaux I, C.N.R.S.-U.R.A. 197, 2, rue du Prof. Jolyet, F-33120 Arcachon, France aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/4(1996-08), 237-250 20:4<237 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford