caa a22 4500 397552505 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00320.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00320.x Diversity of mercury resistance plasmids obtained by exogenous isolation from the bacteria of sugar beet in three successive years [Elektronische Daten] [Andrew K. Lilley, Mark J. Bailey, Martin J. Day, John C. Fry] Self-transmissible plasmids conferring mercury resistance were exogenously isolated from the bacterial populations of sugar beet roots (rhizoplane) and leaves (phyllosphere) into a Pseudomonas putida recipient. Fifty rhizoplane plasmids and 29 phyllosphere plasmids (60-383 kb) were purified. Numerical analysis of plasmid DNA restriction enzyme digest patterns identified five distinct groups. Three of these plasmid groups were isolated from sugar beet crops grown at the same site over three consecutive years, demonstrating their established presence. Each group of plasmids comprised individual isolates with structural additions or deletions. The frequency of exogenous isolation correlated with factors likely to influence plant growth, bacterial activity and the physiological state of donors prior to sampling. All plasmids investigated conferred narrow spectrum mercury resistance with a reductase detoxification mechanism. None of the plasmids conferred resistance to a range of antibiotics, other heavy metals, or to UV, and following transfer to recipient bacteria the range of carbon source utilisation was not altered. This is the first report of the persistence of Pseudomonas spp. plasmid structural types isolated over several years from a terrestrial habitat. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Pseudomonads nationallicence Conjugation nationallicence Plasmid classification nationallicence Horizontal gene transfer nationallicence Mercury resistance nationallicence Lilley Andrew K. Natural Environment Research Council, Molecular Microbial Ecology Group, Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, UK aut Bailey Mark J. Natural Environment Research Council, Molecular Microbial Ecology Group, Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, UK aut Day Martin J. School of Pure and Applied Biology, University of Wales College of Cardiff, P.O. Box 915, Cardiff CF1 3TL, UK aut Fry John C. School of Pure and Applied Biology, University of Wales College of Cardiff, P.O. Box 915, Cardiff CF1 3TL, UK aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/4(1996-08), 211-227 20:4<211 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00320.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00320.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lilley Andrew K. Natural Environment Research Council, Molecular Microbial Ecology Group, Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, UK aut NATIONALLICENCE 700 1- Bailey Mark J. Natural Environment Research Council, Molecular Microbial Ecology Group, Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, UK aut NATIONALLICENCE 700 1- Day Martin J. School of Pure and Applied Biology, University of Wales College of Cardiff, P.O. Box 915, Cardiff CF1 3TL, UK aut NATIONALLICENCE 700 1- Fry John C. School of Pure and Applied Biology, University of Wales College of Cardiff, P.O. Box 915, Cardiff CF1 3TL, UK aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/4(1996-08), 211-227 20:4<211 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford