caa a22 4500 397552521 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00324.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00324.x Isolation and characterization of Methylophaga sulfidovorans sp. nov.: an obligately methylotrophic, aerobic, dimethylsulfide oxidizing bacterium from a microbial mat [Elektronische Daten] [Jolyn M.M. de Zwart, Pieter N. Nelisse, J. Gijs Kuenen] Sediment from a microbial mat from the South-West coast of the Netherlands consumed dimethylsulfide (DMS) under oxic and anoxic conditions. From this sediment, a Gram-negative, oval DMS oxidizing bacterium, strain RB-1, was isolated. Its substrate range is typical of an obligately methylotrophic organism. Enzyme analysis revealed the presence of the ribulose monophosphate pathway for carbon assimilation, and the ability to use the linear dissimilatory pathway via formate to carbon dioxide, as well as the cyclic pathway via the ribulose monophosphate route for carbon dissimilation. 16S rRNA sequence analysis showed high similarity with species belonging to the genus Methylophaga. Because of the specific dimethylsulfide and hydrogen sulfide oxidizing capacity, the new isolate was named Methylophaga sulfidovorans. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Microbial mat nationallicence Dimethylsulfide oxidation nationallicence Methylophaga sulfidovorans nationallicence de Zwart Jolyn M.M. Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands aut Nelisse Pieter N. Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands aut Kuenen J. Gijs Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/4(1996-08), 261-270 20:4<261 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00324.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00324.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Zwart Jolyn M.M. Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands aut NATIONALLICENCE 700 1- Nelisse Pieter N. Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands aut NATIONALLICENCE 700 1- Kuenen J. Gijs Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/4(1996-08), 261-270 20:4<261 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford