caa a22 4500 39755253X CHVBK 20180308164754.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00323.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00323.x Amplification of bacterial DNA bound on clay minerals by the random amplified polymorphic DNA (RAPD) technique [Elektronische Daten] [C. Vettori, D. Paffetti, G. Pietramellara, G. Stotzky, E. Gallori] Chromosomal DNA from Bacillus subtilis, bound on the clay minerals, montmorillonite (Wyoming (W) and Apache County (Ap)) and kaolinite (K), was subjected to the random amplified polymorphic DNA (RAPD) technique. DNA bound on the clays was not amplified with 0.625, 1.875, 6.25, and 12.5 U of Taq DNA polymerase, but amplification occurred when the clay-DNA complexes were diluted 10- and 20-fold or when 21 U of Taq DNA polymerase was added. DNA desorbed from the Ap-DNA and K-DNA equilibrium complexes was amplified with 0.625 U of Taq DNA polymerase, whereas amplification of DNA desorbed from the W-DNA complex occurred only after a 10-fold dilution or when 1.875 U of Taq DNA polymerase was used. These observations indicate that clay minerals differentially affect the amplification process, probably by inhibiting the activity of Taq DNA polymerase. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. RAPD nationallicence Environmental DNA nationallicence Clay-DNA complex nationallicence Vettori C. Department of Animal Biology and Genetics, University of Florence, via Romana 17, 50125 Florence, Italy aut Paffetti D. Department of Animal Biology and Genetics, University of Florence, via Romana 17, 50125 Florence, Italy aut Pietramellara G. Department of Soil Science, University of Florence, Florence, Italy aut Stotzky G. Laboratory of Microbial Ecology, Department of Biology, New York University, New York, USA aut Gallori E. Department of Animal Biology and Genetics, University of Florence, via Romana 17, 50125 Florence, Italy aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/4(1996-08), 251-260 20:4<251 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00323.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00323.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vettori C. Department of Animal Biology and Genetics, University of Florence, via Romana 17, 50125 Florence, Italy aut NATIONALLICENCE 700 1- Paffetti D. Department of Animal Biology and Genetics, University of Florence, via Romana 17, 50125 Florence, Italy aut NATIONALLICENCE 700 1- Pietramellara G. Department of Soil Science, University of Florence, Florence, Italy aut NATIONALLICENCE 700 1- Stotzky G. Laboratory of Microbial Ecology, Department of Biology, New York University, New York, USA aut NATIONALLICENCE 700 1- Gallori E. Department of Animal Biology and Genetics, University of Florence, via Romana 17, 50125 Florence, Italy aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/4(1996-08), 251-260 20:4<251 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford