caa a22 4500 397552548 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00299.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00299.x Influence of temperature on pathways to methane production in the permanently cold profundal sediment of Lake Constance [Elektronische Daten] [Silke Schulz, Ralf Conrad] The in situ temperature of the profundal sediment of Lake Constance is constant at 4°C. Methanogenic bacteria could not be detected at 6°C by the most probable number (MPN) technique using acetate and as methanogenic substrates. Instead, homoacetogenic bacteria were detected on . At a higher temperature of 20°C, however, methanogenic bacteria were found in numbers of about 105 cells ml−1 with and about 5 × 104 cells ml−1 with acetate. However, CH4 production was observed at both 4°C and 20°C. Production of CH4 was inhibited by chloroform and fluoroacetate and the accumulation of intermediary metabolites was measured. At the in situ temperature of 4°C, only acetate accumulated in presence of chloroform. Hydrogen partial pressures were at the same low value of about 0.5 Pa as in the uninhibited control. The amount of accumulated acetate was similar to that of CH4 in the uninhibited controls. Similar results were obtained with fluoroacetate which inhibits methanogenesis from acetate. Addition of H14CO3− did not result in the formation of 14CH4 after 28 days of incubation. However, [2-14C]acetate was immediately converted to 14CH4. The results indicate that methanogenesis at 4°C was exclusively due to acetate cleavage. At 20°C, by contrast, accumulation of H2 was observed in addition to that of acetate, propionate, valerate, caproate, methanol and isopropanol, when CH4 production was inhibited by chloroform. Thermodynamic calculations indicated that the accumulation of the fatty acids was a consequence of feedback inhibition by the accumulated H2. Balance calculations indicated that at 20° about 22% of the CH4 originated from reduction of CO2. Experiments with H14CO3− indicated that about 33% of the CH4 originated from at 20°C. Thermodynamic calculations showed that homoacetogenesis from was endergonic, whereas methanogenesis from acetate or was exergonic at both 4°C and 20°C. Low sediment temperatures obviously limited methanogenesis from for reasons other than thermodynamics. Simultaneously, degradation processes seemed to be dominated by homoacetogenic degradation of organic matter followed by acetoclastic methanogenesis. However, increase of temperature resulted in a dramatic shift of the degradation pathway enhancing the role of H2 as an intermediate. © 1996 Federation of European Microbiological Societies. All rights reserved Temperature nationallicence Homoacetogenesis nationallicence Methanogenesis nationallicence Acetate nationallicence Hydrogen nationallicence Chloroform nationallicence Fluoroacetate nationallicence Gibbs free energy nationallicence Most probable number nationallicence Schulz Silke Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany aut Conrad Ralf Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/1(1996-05), 1-14 20:1<1 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00299.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00299.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schulz Silke Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany aut NATIONALLICENCE 700 1- Conrad Ralf Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/1(1996-05), 1-14 20:1<1 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford