caa a22 4500 397552556 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00301.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00301.x Depth profile of sulfate-reducing bacterial ribosomal RNA and mercury methylation in an estuarine sediment [Elektronische Daten] [Richard Devereux, Michael R. Winfrey, Janet Winfrey, David A. Stahl] The community structure of complex anaerobic microbial communities has been difficult to elucidate because of an inability to cultivate most of the contributing populations. In this study, the distribution of sulfate-reducing bacteria (SRB) in anaerobic sediments was determined using oligonucleotide probes complementary to the 16S ribosomal RNAs of major phylogenetic groups. Sediment cores were collected from Santa Rosa Sound in northwest Florida, and sectioned by depth into 1 to 2 cm fractions. Nucleic acids were extracted from each fraction and hybridized with the SRB-specific ribosomal RNA probes. SRB ribosomal RNAs accounted for almost 5% of the microbial community ribosomal RNA pool in the 3-4 cm depth fraction and were dominated by Desulfovibrionaceae ribosomal RNA. The SRB ribosomal RNA peak coincided with mercury methylation, an activity attributed to SRB. Profiles of the ribosomal RNAs indicate that SRB populations in sediments are stratified by depth. © 1996 Federation of European Microbiological Societies. All rights reserved Sulfate-reducing bacteria nationallicence Mercury methylation nationallicence Ribosomal RNA probes nationallicence Devereux Richard Department of Veterinary Pathobiology and Microbiology, University of Illinois, Urbana, IL 61804, USA aut Winfrey Michael R. Department of Biology and Microbiology, University of Wisconsin-LaCrosse, LaCrosse, WI, USA aut Winfrey Janet Department of Veterinary Pathobiology and Microbiology, University of Illinois, Urbana, IL 61804, USA aut Stahl David A. Department of Veterinary Pathobiology and Microbiology, University of Illinois, Urbana, IL 61804, USA aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/1(1996-05), 23-31 20:1<23 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00301.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00301.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Devereux Richard Department of Veterinary Pathobiology and Microbiology, University of Illinois, Urbana, IL 61804, USA aut NATIONALLICENCE 700 1- Winfrey Michael R. Department of Biology and Microbiology, University of Wisconsin-LaCrosse, LaCrosse, WI, USA aut NATIONALLICENCE 700 1- Winfrey Janet Department of Veterinary Pathobiology and Microbiology, University of Illinois, Urbana, IL 61804, USA aut NATIONALLICENCE 700 1- Stahl David A. Department of Veterinary Pathobiology and Microbiology, University of Illinois, Urbana, IL 61804, USA aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/1(1996-05), 23-31 20:1<23 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford