caa a22 4500 397552572 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00300.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00300.x A demonstration of bacterial conjugation within the alimentary canal of Rhabditis nematodes [Elektronische Daten] [Joseph A. Adamo, Michael A. Gealt] Rhabditis nematodes fed a diet of Escherichia coli defecate viable undigested bacteria. These bacteria retain phenotypic characteristics, including those encoded on plasmids. Nematodes can survive a 2-min surface sterilization with 2% chlorine bleach; internalized bacteria also survive this treatment and are released in the nematode wastes. Bacteria alone or on the surface of dead nematodes are unable to survive incubation with this solution. There were 3.2 × 105 viable bacteria per nematode, indicating that sufficient bacteria were present for gene transfer. Transconjugants (lac−nalRstrRcmR) were recovered in the nematode fecal material following a protocol where nematodes were initially fed a plasmidless lac−nalRstrScmSE. coli and then, after surface sterilization, a lac+nalSE. coli plasmid donor containing the conjugative R100JA (strRcmR) plasmid. The presence of plasmids in the transconjugants was confirmed by gel electrophoresis. The occurrence of conjugation in the gut was confirmed by dissection of individual surface-sterilized nematodes and isolation of transconjugants. © 1996 Federation of European Microbiological Societies. All rights reserved Bacterial conjugation nationallicence Nematodes nationallicence Environmental microbiology nationallicence Invertebrates nationallicence Gene transfer nationallicence Adamo Joseph A. Science Department, Ocean County College, Toms River, New Jersey, NJ 08753, USA aut Gealt Michael A. Department of Bioscience and Biotechnology and Environmental Studies Institute, Drexel University, 32nd & Chestnut Streets, Philadelphia, PA 19104, USA aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/1(1996-05), 15-22 20:1<15 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00300.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00300.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adamo Joseph A. Science Department, Ocean County College, Toms River, New Jersey, NJ 08753, USA aut NATIONALLICENCE 700 1- Gealt Michael A. Department of Bioscience and Biotechnology and Environmental Studies Institute, Drexel University, 32nd & Chestnut Streets, Philadelphia, PA 19104, USA aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/1(1996-05), 15-22 20:1<15 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford