caa a22 4500 397552610 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00214.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00214.x Rhizosphere competence of fluorescent Pseudomonas sp. B24 genetically modified to utilise additional ferric siderophores [Elektronische Daten] [Yvan Moënne-Loccoz, Brendan McHugh, Peter M. Stephens, Fiona I. McConnell, Jeremy D. Glennon, David N. Dowling, Fergal O'Gara] The ability to utilise additional siderophores may increase the ecological fitness of biocontrol inoculants of Pseudomonas in the rhizosphere. Plasmid pCUP2 carries a copy of the gene pbuA coding for the membrane receptor of ferric pseudobactin M114. Pseudomonas sp. B24Rif containing pCUP2 can utilise ferric pseudobactin of P. fluorescens M114 in addition to its own siderophore. A larger fraction of the culturable resident fluorescent pseudomonads in the rhizosphere of sugarbeet grown in a low-iron sandy loam soil could supply siderophore-complexed iron to B24Rif(pCUP2) rather than to B24Rif. However, B24Rif and B24Rif(pCUP2) were found at similar population levels in the rhizosphere for 21 days after their inoculation on seeds. A total of 25 of 43 isolates of resident fluorescent Pseudomonas unable to cross-feed iron to B24Rif could cross-feed B24Rif(pCUP2) and they were subdivided into seven different strains by arbitrary-primed PCR fingerprinting. The siderophores produced by 11 of them were typed by HPLC and they were similar to pseudobactin M114. However, the ability to utilise ferric pseudobactin M114 did not improve the ecological fitness of B24Rif in the rhizosphere of sugarbeet although a larger fraction of the culturable resident fluorescent pseudomonads could supply pseudobactin M114-complexed iron to B24Rif(pCUP2) than to B24Rif. © 1996 Federation of European Microbiological Societies. All rights reserved Pseudomonas nationallicence Siderophore nationallicence ApPCR nationallicence Rhizosphere colonisation nationallicence Moënne-Loccoz Yvan Microbiology Department, University College Cork, Cork, Ireland aut McHugh Brendan Microbiology Department, University College Cork, Cork, Ireland aut Stephens Peter M. Microbiology Department, University College Cork, Cork, Ireland aut McConnell Fiona I. Microbiology Department, University College Cork, Cork, Ireland aut Glennon Jeremy D. Chemistry Department, University College Cork, Cork, Ireland aut Dowling David N. Microbiology Department, University College Cork, Cork, Ireland aut O'Gara Fergal Microbiology Department, University College Cork, Cork, Ireland aut FEMS Microbiology Ecology Blackwell Publishing Ltd 19/4(1996-04), 215-225 19:4<215 1996 19 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00214.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00214.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moënne-Loccoz Yvan Microbiology Department, University College Cork, Cork, Ireland aut NATIONALLICENCE 700 1- McHugh Brendan Microbiology Department, University College Cork, Cork, Ireland aut NATIONALLICENCE 700 1- Stephens Peter M. Microbiology Department, University College Cork, Cork, Ireland aut NATIONALLICENCE 700 1- McConnell Fiona I. Microbiology Department, University College Cork, Cork, Ireland aut NATIONALLICENCE 700 1- Glennon Jeremy D. Chemistry Department, University College Cork, Cork, Ireland aut NATIONALLICENCE 700 1- Dowling David N. Microbiology Department, University College Cork, Cork, Ireland aut NATIONALLICENCE 700 1- O'Gara Fergal Microbiology Department, University College Cork, Cork, Ireland aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 19/4(1996-04), 215-225 19:4<215 1996 19 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford