caa a22 4500 397552629 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00216.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00216.x Metabolism of methylated osmolytes by aerobic bacteria from Mono Lake, a moderately hypersaline, alkaline environment [Elektronische Daten] [Mara R. Diaz, Barrie F. Taylor] Three strains of aerobic bacteria were isolated from water and sediment samples of Mono Lake, a moderately hypersaline (90 ppt), alkaline (pH 9.7) lake in California. The organisms, Gram-negative rods, grew fastest at about pH 9.7 with no growth or much slower growth at pH 7.0. All three isolates grew on glycine betaine (GB) and respirometric experiments indicated that catabolism was by sequential demethylation with dimethyl glycine and sarcosine as intermediates. Two of the isolates also grew on dimethylsulfoniopropionate (DMSP), one with cleavage of the DMSP to yield dimethyl sulfide (DMS) and acrylate, and the other by demethylation with 3-methiolpropionate (MMPA) as an intermediate and the production of methanethiol from MMPA. The methylated osmolytes supported growth at salinities similar to those in Mono Lake, but, at higher salinities, catabolism was suppressed and GB and DMSP functioned as osmolytes. GB and DMSP probably originate from cyanobacteria and/or phytoplankton in Mono Lake and this report is the first indication of both the DMS and demethylation/methanethiol-producing pathways for DMSP degradation in a nonmarine environment. © 1996 Federation of European Microbiological Societies. All rights reserved Dimethylsulfoniopropionate (DMSP) nationallicence 3-Mercaptopropionate (MPA) nationallicence 3-Methiolpropionate (MMPA) nationallicence Glycine betaine (GB) nationallicence Compatible solutes nationallicence Mono Lake nationallicence Diaz Mara R. Division of Marine and Atmospheric Chemistry, Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, FL 33149-1098, USA aut Taylor Barrie F. Division of Marine and Atmospheric Chemistry, Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, FL 33149-1098, USA aut FEMS Microbiology Ecology Blackwell Publishing Ltd 19/4(1996-04), 239-247 19:4<239 1996 19 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00216.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00216.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Diaz Mara R. Division of Marine and Atmospheric Chemistry, Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, FL 33149-1098, USA aut NATIONALLICENCE 700 1- Taylor Barrie F. Division of Marine and Atmospheric Chemistry, Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, FL 33149-1098, USA aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 19/4(1996-04), 239-247 19:4<239 1996 19 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford