caa a22 4500 397552661 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00215.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00215.x Bååth Erland Department of Microbial Ecology, Ecology Building, Lund University, S-223 62 Lund, Sweden Adaptation of soil bacterial communities to prevailing pH in different soils [Elektronische Daten] [Erland Bååth] The bacterial community response to pH was studied for 16 soils with pH(H2O) ranging between 4 and 8 by measuring thymidine incorporation into bacteria extracted from the soil into a solution using homogenization-centrifugation. The pH of the bacterial solution was altered to six different values with dilute sulfuric acid or different buffers before measuring incorporation. The resulting pH response curve for thymidine incorporation was used to compare bacterial communities from the different soils. There was a correlation between optimum pH for thymidine incorporation and the soil pH(H2O). Even bacterial communities from acid soils had optima corresponding to the soil pH, indicating that they were adapted to these conditions. Thymidine incorporation was also compared with leucine incorporation for some soils. The leucine to thymidine incorporation ratio was constant over the tested pH interval when incorporation values were adjusted for isotope dilution. A good correlation was found between the scores along the first component (explaining 80% of the variation) and soil pH (r2 = 0.85), if principal component analysis of the pH response curves for thymidine incorporation was used. The pH response curves differed most for the extreme pH values used, and a linear relationship was found between the logarithm of the ratio of thymidine incorporation at pH 4.3 to incorporation at pH 8.2 and the soil pH (r2 = 0.86). Thus, a simplified technique using only two pH values, when measuring the thymidine incorporation, could be used to compare the response to pH of bacterial communities. © 1996 Federation of European Microbiological Societies. All rights reserved Soil nationallicence pH response nationallicence Thymidine incorporation nationallicence Bacterial community nationallicence FEMS Microbiology Ecology Blackwell Publishing Ltd 19/4(1996-04), 227-237 19:4<227 1996 19 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00215.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00215.x text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Bååth Erland Department of Microbial Ecology, Ecology Building, Lund University, S-223 62 Lund, Sweden NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 19/4(1996-04), 227-237 19:4<227 1996 19 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford