caa a22 4500 397552726 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00212.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00212.x Acetogenesis from H2 and CO2 by methane- and non-methane-producing human colonic bacterial communities [Elektronische Daten] [Annick Bernalier, Marielle Lelait, Violaine Rochet, Jean-Philippe Grivet, Glenn R. Gibson, Michelle Durand] The purpose of the study was to define the potential for reductive acetogenesis of colonic microflora from six non\u2010methane\u2010 and four methane\u2010excreting human subjects in relation to numbers of the different H2\u2010utilizing microorganisms. Faecal bacterial suspensions were incubated in the presence of NaH13CO3 and under a gas phase composed of either 100% N2 (control) or 80% H2\u201320% N2. The effects of a specific methanogenesis inhibitor or of sulfate supplementation were also determined. Quantitative nuclear magnetic resonance showed the presence of both single\u2010 and double\u2010labelled acetate in all incubations under hydrogen. H2/CO2\u2010acetogenesis appears to be a quantitatively important activity only in the presence of very low numbers of methanogens. Inhibition of methanogenesis induced a large increase in 13CO2 incorporation into acetate in CH4\u2010producing samples. These results showed that methanogens can efficiently outcompete acetogens in human colonic contents. In contrast, no clear\u2010cut competition for H2 between acetogenesis and dissimilatory sulfate\u2010reduction could be demonstrated. A slight reduction of the acetogenic activity was only observed at the highest sulfate addition (100 mM). © 1996 Federation of European Microbiological Societies. All rights reserved Reductive acetogenesis nationallicence Human faeces nationallicence 13C\u2010NMR nationallicence Hydrogen nationallicence Interrelationships nationallicence Bernalier Annick Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France aut Lelait Marielle Centre de Biophysique Moléculaire, CNRS and Université d'Orléans, 1A Avenue de la Recherche, 45071 Orléans cedex 02, France aut Rochet Violaine Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France aut Grivet Jean-Philippe Centre de Biophysique Moléculaire, CNRS and Université d'Orléans, 1A Avenue de la Recherche, 45071 Orléans cedex 02, France aut Gibson Glenn R. MRC, Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK aut Durand Michelle Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France aut FEMS Microbiology Ecology Blackwell Publishing Ltd 19/3(1996-03), 193-202 19:3<193 1996 19 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00212.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00212.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bernalier Annick Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France aut NATIONALLICENCE 700 1- Lelait Marielle Centre de Biophysique Moléculaire, CNRS and Université d'Orléans, 1A Avenue de la Recherche, 45071 Orléans cedex 02, France aut NATIONALLICENCE 700 1- Rochet Violaine Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France aut NATIONALLICENCE 700 1- Grivet Jean-Philippe Centre de Biophysique Moléculaire, CNRS and Université d'Orléans, 1A Avenue de la Recherche, 45071 Orléans cedex 02, France aut NATIONALLICENCE 700 1- Gibson Glenn R. MRC, Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK aut NATIONALLICENCE 700 1- Durand Michelle Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 19/3(1996-03), 193-202 19:3<193 1996 19 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford