caa a22 4500 397552769 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00346.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00346.x Diel variability in transcription of the structural gene for glutamine synthetase (glnA) in natural populations of the marine diazotrophic cyanobacterium Trichodesmium thiebautii [Elektronische Daten] [Jonathan G. Kramer, Michael Wyman, Jonathan P. Zehr, Douglas G. Capone] A 360-bp fragment of the Trichodesmium thiebautii glutamine synthetase gene (glnA) was amplified from DNA isolated from oceanic populations of this ecologically important, diazotrophic cyanobacterium. The cloned fragment showed high homology to the type 1 glutamine synthetase (GS) enzymes of other cyanobacteria and was used to probe the in situ, temporal variability in transcription of Trichodesmium GS. Three distinct phases in the diel pattern of glnA mRNA abundance were observed. These appear to be correlated with the temporal variability in carbon and nitrogen fixation rates and the effect that these physiological processes have on the size of cellular pools of the primary end-products of N assimilation. Our data show that Trichodesmium thiebautii GS is regulated at the level of transcription and support the hypothesis that GS expression in this organism is subject to similar controls as those established for heterocyst-forming cyanobacteria. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Trichodesmium glutamine synthetase nationallicence glnA gene expression nationallicence Diel variability of natural populations nationallicence Kramer Jonathan G. Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Columbus Center, Baltimore, MD 21202, USA aut Wyman Michael Plymouth Marine Laboratory, Citadel Hill, Plymouth, UK aut Zehr Jonathan P. Biology Department, Rensselaer Polytechnic Inst., Troy, NY 12180, USA aut Capone Douglas G. Chesapeake Biological Laboratory, Center for Environmental and Estuarine Studies, University of Maryland, Solomons, MD 20688, USA aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/3(1996-11), 187-196 21:3<187 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00346.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00346.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kramer Jonathan G. Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Columbus Center, Baltimore, MD 21202, USA aut NATIONALLICENCE 700 1- Wyman Michael Plymouth Marine Laboratory, Citadel Hill, Plymouth, UK aut NATIONALLICENCE 700 1- Zehr Jonathan P. Biology Department, Rensselaer Polytechnic Inst., Troy, NY 12180, USA aut NATIONALLICENCE 700 1- Capone Douglas G. Chesapeake Biological Laboratory, Center for Environmental and Estuarine Studies, University of Maryland, Solomons, MD 20688, USA aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/3(1996-11), 187-196 21:3<187 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford