caa a22 4500 397552777 CHVBK 20180308164754.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00345.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00345.x Dissimilatory nitrite-reductase provides a competitive advantage to Pseudomonas sp. RTC01 to colonise the centre of soil aggregates [Elektronische Daten] [L. Philippot, P. Renault, J. Sierra, C. Hénault, A. Clays-Josserand, C. Chenu, R. Chaussod, R. Lensi] The effect of soil aggregation on denitrification has been studied in different ways: artificial or natural aggregates individually analysed or comparison of denitrifying activity of different size classes of aggregates. However, until now, no work has been conducted to evaluate the role of denitrification in the microbial colonisation of soil aggregates. Over a one-month period, we examined on remoulded nonsterile soil aggregates the survival of inoculated wild-type Pseudomonas strain and of its corresponding isogenic Tn5 mutant (Nir−) lacking the ability to synthesize the dissimilative nitrite-reductase. Simultaneously, the evolution of the O2 repartition inside the soil aggregates was assessed by the use of microelectrodes. The inner and outer portions were roughly anoxic and oxic, respectively, during the entire experiment while the intermediate portion showed fluctuating aeration conditions. The values of the Nir− to wild-type + Nir− ratio were found in the following order: inner < intermediate < outer portion, demonstrating that the nitrite-reductase may provide a competitive advantage to the Pseudomonas strain to colonise the centre of soil aggregates. However, a clear differentiation between inner and outer aggregates portions was not observed with the indigenous microflora (denitrifiers-to-total heterotrophs, biomass or physiological abilities). © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Denitrification nationallicence Nitrite-reductase nationallicence Aggregate nationallicence Pseudomonas sp nationallicence Oxygen profile nationallicence Philippot L. UNR CNRS 5557, Laboratoire d'Ecologie Microbienne du Sol, 43 Blvd. du 11 Novembre 1918, Université Claude Bernard- Lyon I, 69622 Villeurbanne Cedex, France aut Renault P. INRA, Unité de Science du Sol, Domaine Saint-Paul, Site AgroParc, 84914 Avignon Cedex 9, France aut Sierra J. INRA, Unité de Science du Sol, Domaine Saint-Paul, Site AgroParc, 84914 Avignon Cedex 9, France aut Hénault C. INRA, Laboratoire de Microbiologie des Sols, 17 rue Sully, BP 1540, 21034 Dijon Cedex, France aut Clays-Josserand A. UNR CNRS 5557, Laboratoire d'Ecologie Microbienne du Sol, 43 Blvd. du 11 Novembre 1918, Université Claude Bernard- Lyon I, 69622 Villeurbanne Cedex, France aut Chenu C. INRA, Unité de Science du Sol, Route de Saint-Cyr, 78026 Versailles Cedex, France aut Chaussod R. INRA, Laboratoire de Microbiologie des Sols, 17 rue Sully, BP 1540, 21034 Dijon Cedex, France aut Lensi R. UNR CNRS 5557, Laboratoire d'Ecologie Microbienne du Sol, 43 Blvd. du 11 Novembre 1918, Université Claude Bernard- Lyon I, 69622 Villeurbanne Cedex, France aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/3(1996-11), 175-185 21:3<175 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00345.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00345.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Philippot L. UNR CNRS 5557, Laboratoire d'Ecologie Microbienne du Sol, 43 Blvd. du 11 Novembre 1918, Université Claude Bernard- Lyon I, 69622 Villeurbanne Cedex, France aut NATIONALLICENCE 700 1- Renault P. INRA, Unité de Science du Sol, Domaine Saint-Paul, Site AgroParc, 84914 Avignon Cedex 9, France aut NATIONALLICENCE 700 1- Sierra J. INRA, Unité de Science du Sol, Domaine Saint-Paul, Site AgroParc, 84914 Avignon Cedex 9, France aut NATIONALLICENCE 700 1- Hénault C. INRA, Laboratoire de Microbiologie des Sols, 17 rue Sully, BP 1540, 21034 Dijon Cedex, France aut NATIONALLICENCE 700 1- Clays-Josserand A. UNR CNRS 5557, Laboratoire d'Ecologie Microbienne du Sol, 43 Blvd. du 11 Novembre 1918, Université Claude Bernard- Lyon I, 69622 Villeurbanne Cedex, France aut NATIONALLICENCE 700 1- Chenu C. INRA, Unité de Science du Sol, Route de Saint-Cyr, 78026 Versailles Cedex, France aut NATIONALLICENCE 700 1- Chaussod R. INRA, Laboratoire de Microbiologie des Sols, 17 rue Sully, BP 1540, 21034 Dijon Cedex, France aut NATIONALLICENCE 700 1- Lensi R. UNR CNRS 5557, Laboratoire d'Ecologie Microbienne du Sol, 43 Blvd. du 11 Novembre 1918, Université Claude Bernard- Lyon I, 69622 Villeurbanne Cedex, France aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/3(1996-11), 175-185 21:3<175 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford