caa a22 4500 397552793 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00343.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00343.x Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms [Elektronische Daten] [Sylvie Clerc, Pascal Simonet] The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Conjugation nationallicence Streptomyces nationallicence Plasmid pSAM2 nationallicence Soil microcosm nationallicence Clerc Sylvie Laboratoire d'Ecologie Microbienne du sol, UMR CNRS 5557, Université Lyon I, 43 Boulevard du 11 novembre 1918, 69622 Villeurbanne Cedex, France aut Simonet Pascal Laboratoire d'Ecologie Microbienne du sol, UMR CNRS 5557, Université Lyon I, 43 Boulevard du 11 novembre 1918, 69622 Villeurbanne Cedex, France aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/3(1996-11), 157-165 21:3<157 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00343.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00343.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clerc Sylvie Laboratoire d'Ecologie Microbienne du sol, UMR CNRS 5557, Université Lyon I, 43 Boulevard du 11 novembre 1918, 69622 Villeurbanne Cedex, France aut NATIONALLICENCE 700 1- Simonet Pascal Laboratoire d'Ecologie Microbienne du sol, UMR CNRS 5557, Université Lyon I, 43 Boulevard du 11 novembre 1918, 69622 Villeurbanne Cedex, France aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/3(1996-11), 157-165 21:3<157 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford