caa a22 4500 397552823 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00347.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00347.x Methane oxidation potential and preliminary analysis of methanotrophs in blanket bog peat using molecular ecology techniques [Elektronische Daten] [Ian R. McDonald, Graham H. Hall, Roger W. Pickup, J. Colin Murrell] The potential for methane oxidation was measured, and methanotroph gene sequences studied, in a peat core from the Moorhouse Nature Reserve, UK. Methane oxidation potential was observed in all depths of the peat core (down to 30 cm), and was inhibited by addition of acetylene, indicating the involvement of methane-oxidising bacteria. A peak of activity was shown in the 10-12 cm horizon, below which activity decreased with depth. Above this horizon, methane oxidation was relatively high and showed little change with depth. 16S rDNA libraries from several sections of the peat core were screened with methanotroph 16S rDNA probes designed to detect the genera Methylomonas, Methylococcus, Methylobacter and Methylosinus. Two clones, MHP14 and MHP17, hybridised strongly with the Methylosinus probe and upon complete sequencing and phylogenetic analysis were shown to group closely to the Methylosinus/Methylocystis genera of methanotrophs. However, the clones do form a distinct branch of their own, supported by BOOTSTRAP values, and may represent a novel group of acidophilic methanotrophs which have yet to be cultured. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. 16S rRNA nationallicence Methane oxidation potential nationallicence Peat nationallicence PCR nationallicence Soluble methane monooxygenase nationallicence Phylogeny nationallicence McDonald Ian R. Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK aut Hall Graham H. Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria LA22 0LP, UK aut Pickup Roger W. Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria LA22 0LP, UK aut Murrell J. Colin Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/3(1996-11), 197-211 21:3<197 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00347.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00347.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McDonald Ian R. Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK aut NATIONALLICENCE 700 1- Hall Graham H. Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria LA22 0LP, UK aut NATIONALLICENCE 700 1- Pickup Roger W. Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria LA22 0LP, UK aut NATIONALLICENCE 700 1- Murrell J. Colin Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/3(1996-11), 197-211 21:3<197 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford