caa a22 4500 397552874 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00337.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00337.x Rapid and sensitive detection by PCR of mpcI gene in the rhizosphere [Elektronische Daten] [Sung-Cheol Koh, Petra Marschner, David E. Crowley, Dennis D. Focht] A simple and sensitive detection system, using polymerase chain reaction (PCR) and a soil microcosm, was developed to detect a bacterial catabolic gene in the rhizosphere. The inoculated population of Alcaligenes eutrophus JMP134, a phenol and 2,4-dichlorophenoxy acetic acid utilizer, was readily detected by this technique, which permitted taking of samples from specific locations of root (including rhizosphere) and soil. The number of JMP134 viable cells (102-103 cells), typically picked up by the nitrocellulose filter strip method, yielded sufficient amount of the target DNA to be detected by PCR. Primers encoding metapyrocatechase I (MPC I; catechol 2,3-dioxygenase) enabled the discrimination of at least five viable cells of JMP134 among the indigenous microorganisms inhabiting bush bean roots. This simplified PCR detection procedure facilitated monitoring of the specific degradative gene in the rhizosphere in only 5 h. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Metapyrocatechase nationallicence Polymerase chain reaction nationallicence Rhizosphere nationallicence Soil microcosm nationallicence 2,4-Dichlorophenoxy acetic acid nationallicence Koh Sung-Cheol Department of Soil and Environmental Sciences, University of California, Riverside, CA 92521, USA aut Marschner Petra Department of Soil and Environmental Sciences, University of California, Riverside, CA 92521, USA aut Crowley David E. Department of Soil and Environmental Sciences, University of California, Riverside, CA 92521, USA aut Focht Dennis D. Department of Soil and Environmental Sciences, University of California, Riverside, CA 92521, USA aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/2(1996-10), 103-108 21:2<103 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00337.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00337.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koh Sung-Cheol Department of Soil and Environmental Sciences, University of California, Riverside, CA 92521, USA aut NATIONALLICENCE 700 1- Marschner Petra Department of Soil and Environmental Sciences, University of California, Riverside, CA 92521, USA aut NATIONALLICENCE 700 1- Crowley David E. Department of Soil and Environmental Sciences, University of California, Riverside, CA 92521, USA aut NATIONALLICENCE 700 1- Focht Dennis D. Department of Soil and Environmental Sciences, University of California, Riverside, CA 92521, USA aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/2(1996-10), 103-108 21:2<103 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford