caa a22 4500 397552890 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199610 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00338.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00338.x Pine and spruce seedling growth and mycorrhizal infection after inoculation with plant growth promoting Pseudomonas strains [Elektronische Daten] [Masahiro Shishido, Daniel J. Petersen, Hugues B. Massicotte, Christopher P. Chanway] Pine and spruce seeds were inoculated with antibiotic-resistant plant growth promoting fluorescent Pseudomonas strains Sm3-RN, Ss2-RN and Sw5-RN for evaluation of bacterial root colonization and seedling growth responses under greenhouse conditions. Mycorrhizal inoculum was introduced to seedling containers by placing 2 cc of forest floor soil around seeds at the time of sowing. Mycorrhizal roots were detected on 39% of pine and 30% of spruce seedlings treated with forest soil 13-15 weeks later. Most mycorrhizae were formed by Wilcoxina sp. (E-strain) (89% for spruce and 69% for pine); some Amphinema-like, Mycelium radicis atrovirens, Suillus-like, Thelephora-like, and Tuber-like mycorrhizae were also detected. In the absence of bacterial inoculum, spruce seedling biomass was positively correlated with the number of mycorrhizal root tips. This trend was obscured following inoculation with strains Ss2-RN and Sw5-RN. Pine seedling growth was weakly correlated with mycorrhizal root tip development only after treatment with strain Sm3-RN. Bacterial inoculation did not influence the mycorrhizal status of seedlings, but all three Pseudomonas strains stimulated biomass accumulation of spruce and pine seedlings, up to 19% (P < 0.05). Non-mycorrhizal seedlings tended to support smaller root-associated Pseudomonas populations, but inoculant bacteria colonized seedlings with a minimum of 6.3 × 103 colony forming units per gram of rhizosphere soil regardless of their mycorrhizal status. For spruce, growth effects due to bacterial inoculation were similar in both mycorrhizal and non-mycorrhizal seedlings. However, statistically significant gains in pine biomass by inoculation with strains Sm3-RN and Ss2-RN occurred only in mycorrhizal seedlings, whereas strain Sw5-RN caused significant growth promotion only in non-mycorrhizal pine. Our results suggest that these fluorescent pseudomonad strains enhanced spruce seedling growth through mechanisms unrelated to increased mycorrhizal colonization, but growth promotion of pine by strains Sm3-RN and Ss2-RN was facilitated by an interaction with mycorrhizae. © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. Inoculation nationallicence Pseudomonas nationallicence Conifer seedling growth promotion nationallicence Mycorrhizae nationallicence Shishido Masahiro Department of Forest Sciences, University of British Columbia, Suite 270-2357 Main Mall, Vancouver, BC V6T 1Z4, Canada aut Petersen Daniel J. Department of Forest Sciences, University of British Columbia, Suite 270-2357 Main Mall, Vancouver, BC V6T 1Z4, Canada aut Massicotte Hugues B. Faculty of Natural Resources and Environmental Studies, University of Northern British Columbia, Prince George, BC V2N 4Z9, Canada aut Chanway Christopher P. Department of Forest Sciences, University of British Columbia, Suite 270-2357 Main Mall, Vancouver, BC V6T 1Z4, Canada aut FEMS Microbiology Ecology Blackwell Publishing Ltd 21/2(1996-10), 109-119 21:2<109 1996 21 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00338.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00338.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shishido Masahiro Department of Forest Sciences, University of British Columbia, Suite 270-2357 Main Mall, Vancouver, BC V6T 1Z4, Canada aut NATIONALLICENCE 700 1- Petersen Daniel J. Department of Forest Sciences, University of British Columbia, Suite 270-2357 Main Mall, Vancouver, BC V6T 1Z4, Canada aut NATIONALLICENCE 700 1- Massicotte Hugues B. Faculty of Natural Resources and Environmental Studies, University of Northern British Columbia, Prince George, BC V2N 4Z9, Canada aut NATIONALLICENCE 700 1- Chanway Christopher P. Department of Forest Sciences, University of British Columbia, Suite 270-2357 Main Mall, Vancouver, BC V6T 1Z4, Canada aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 21/2(1996-10), 109-119 21:2<109 1996 21 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford