caa a22 4500 397552920 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00311.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00311.x Detection and quantification of degradative genes in soils contaminated by toluene [Elektronische Daten] [Sylvie Hallier-Soulier, Véronique Ducrocq, Nathalie Mazure, Nicole Truffaut] A method based on the polymerase chain reaction (PCR) was developed for a rapid and specific detection of toluene degradative genes in soil. The xylE gene coding for catechol 2,3-dioxygenase was chosen as a target gene. The detection threshold was evaluated in microcosms using a sterilized standard soil inoculated with various amounts of a degradative strain of Pseudomonas putida (mX). The extracted DNA was used as a template to amplify the xylE gene. PCR followed by hybridization with an internal probe allowed us to detect 102 bacteria per g of soil. In polluted soils, quantification of target DNA by competitive PCR was compared with enumeration of degradative microflora. This molecular method appeared to be rapid, sensitive and more suitable than the microbiological approach to estimate the biodegradative potential of a polluted soil. © 1996 Federation of European Microbiological Societies. All rights reserved Toluene biodegradation nationallicence xylE gene nationallicence Soil microcosm nationallicence Polymerase chain reaction nationallicence Hallier-Soulier Sylvie Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, BP 649, 60206 Compiègne Cedex, France aut Ducrocq Véronique Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, BP 649, 60206 Compiègne Cedex, France aut Mazure Nathalie Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, BP 649, 60206 Compiègne Cedex, France aut Truffaut Nicole Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, BP 649, 60206 Compiègne Cedex, France aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/2(1996-06), 121-133 20:2<121 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00311.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00311.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hallier-Soulier Sylvie Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, BP 649, 60206 Compiègne Cedex, France aut NATIONALLICENCE 700 1- Ducrocq Véronique Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, BP 649, 60206 Compiègne Cedex, France aut NATIONALLICENCE 700 1- Mazure Nathalie Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, BP 649, 60206 Compiègne Cedex, France aut NATIONALLICENCE 700 1- Truffaut Nicole Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, BP 649, 60206 Compiègne Cedex, France aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/2(1996-06), 121-133 20:2<121 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford