caa a22 4500 397552955 CHVBK 20180308164755.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1111/j.1574-6941.1996.tb00308.x doi (NATIONALLICENCE)oxford-10.1111/j.1574-6941.1996.tb00308.x Evaluation of an rRNA-targeted oligonucleotide probe for the detection of mosquitocidal strains of Bacillus sphaericus in soils: characterization of novel strains lacking toxin genes [Elektronische Daten] [Ulrich Jahnz, Alisdair Fitch, Fergus G. Priest] Colonies of Bacillus sphaericus on primary isolation have been identified using an oligonucleotide probe targeted to a specific region of the 16S rRNA. Of 3440 colonies from soil samples from Brazil, 57 hybridized to the probe in colony blots but when purified DNA was used in slot-blots the probe was more specific and only 27 isolates hybridized. Of these, 20 strains were confirmed as members of DNA homology group IIA (potential mosquitocidal strains) by ribotyping and isoenzyme analysis. However, none of these strains was toxic to Anopheles or Culex larvae, nor did they contain recognized toxin genes. This is the first demonstration of such non-pathogenic strains of B. sphaericus DNA homology group IIA and their common occurrence suggests that pathogenicity is not an important contribution to the success of these bacteria in the environment. Similar screening of strains from Scottish soils indicated that B. sphaericus DNA homology group IIA strains were less common in this habitat and none were recovered on this occasion. © 1996 Federation of European Microbiological Societies. All rights reserved Bacillus sphaericus nationallicence Mosquitocidal toxins nationallicence Oligonucleotide probe nationallicence Jahnz Ulrich Department of Biological Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK aut Fitch Alisdair Department of Biological Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK aut Priest Fergus G. Department of Biological Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK aut FEMS Microbiology Ecology Blackwell Publishing Ltd 20/2(1996-06), 91-99 20:2<91 1996 20 femsec https://doi.org/10.1111/j.1574-6941.1996.tb00308.x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1111/j.1574-6941.1996.tb00308.x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jahnz Ulrich Department of Biological Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK aut NATIONALLICENCE 700 1- Fitch Alisdair Department of Biological Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK aut NATIONALLICENCE 700 1- Priest Fergus G. Department of Biological Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK aut NATIONALLICENCE 773 0- FEMS Microbiology Ecology Blackwell Publishing Ltd 20/2(1996-06), 91-99 20:2<91 1996 20 femsec Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford